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Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using SLC25A12 Monoclonal antibody (67467-1-Ig)

    WB analysis of LNCaP using 67467-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67467-1-Ig (SLC25A12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using SLC25A12 Monoclonal antibody (67467-1-Ig)

    WB analysis of Jurkat using 67467-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67467-1-Ig (SLC25A12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using SLC25A12 Monoclonal antibody (67467-1-Ig)

    WB analysis of A431 using 67467-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 67467-1-Ig (SLC25A12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC25A12 Monoclonal antibody (67467-1-Ig)

    IHC staining of mouse brain using 67467-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC25A12 Monoclonal antibody (67467-1-Ig)

    IHC staining of mouse brain using 67467-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SLC25A12 Monoclonal antibody (67467-1-Ig)

    IHC staining of mouse kidney using 67467-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SLC25A12 Monoclonal antibody (67467-1-Ig)

    IHC staining of mouse kidney using 67467-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SLC25A12 Monoclonal antibody (67467-1-Ig)

    IHC staining of human breast cancer using 67467-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SLC25A12 Monoclonal antibody (67467-1-Ig)

    IHC staining of human breast cancer using 67467-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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SLC25A12 Monoclonal antibody

SLC25A12 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67467-1-Ig
Clone No.1F2B10
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Synonyms

AGC1, Araceli hiperlarga, Aralar, Aralar1, Electrogenic aspartate/glutamate antiporter SLC25A12, mitochondrial

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, A431 cells, Jurkat cells
Positive IHC detected inmouse brain tissue, human breast cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

67467-1-Ig targets SLC25A12 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SLC25A12 fusion protein Ag13576 相同性解析による交差性が予測される生物種
Full Name solute carrier family 25 (mitochondrial carrier, Aralar), member 12
Calculated molecular weight 75 kDa, 63 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC016932
Gene Symbol SLC25A12
Gene ID (NCBI) 8604
RRIDAB_2882696
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75746
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SLC25A12 antibody 67467-1-IgDownload protocol
IHC protocol for SLC25A12 antibody 67467-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Nat Commun

Decoding the mechanical characteristics of the human anterior cruciate ligament entheses through graduated mineralization interfaces

Authors - Jinghua Fang
View Article