Validation Data Gallery
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Tested Applications
| Positive WB detected in | LNCaP cells, A431 cells, Jurkat cells |
| Positive IHC detected in | mouse brain tissue, human breast cancer tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
67467-1-Ig targets SLC25A12 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SLC25A12 fusion protein Ag13576 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 25 (mitochondrial carrier, Aralar), member 12 |
| Calculated molecular weight | 75 kDa, 63 kDa |
| Observed molecular weight | 70-75 kDa |
| GenBank accession number | BC016932 |
| Gene Symbol | SLC25A12 |
| Gene ID (NCBI) | 8604 |
| RRID | AB_2882696 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O75746 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SLC25A12, also known as ARALAR1, is a key component of the malate-aspartate shuttle, a metabolic system that facilitates the transfer of reducing equivalents (NADH) from the cytoplasm into mitochondria, thereby supporting oxidative phosphorylation and ATP production. SLC25A12 dysfunction results in impaired mitochondrial energy production, which can lead to neuronal damage, developmental delays, and myelination defects (PMID: 20015484).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC25A12 antibody 67467-1-Ig | Download protocol |
| IHC protocol for SLC25A12 antibody 67467-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Decoding the mechanical characteristics of the human anterior cruciate ligament entheses through graduated mineralization interfaces |