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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC25A12 Polyclonal antibody (26804-1-AP)

    WB analysis using 26804-1-AP (same clone as 26804-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26804-1-AP (SLC25A12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26804-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SLC25A12 Polyclonal antibody (26804-1-AP)

    IHC staining of human breast cancer using 26804-1-AP (same clone as 26804-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26804-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SLC25A12 Polyclonal antibody (26804-1-AP)

    IHC staining of human breast cancer using 26804-1-AP (same clone as 26804-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26804-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SLC25A12 Polyclonal antibody (26804-1-AP)

    IHC staining of human pancreas cancer using 26804-1-AP (same clone as 26804-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26804-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SLC25A12 Polyclonal antibody (26804-1-AP)

    IHC staining of human pancreas cancer using 26804-1-AP (same clone as 26804-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26804-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SLC25A12 Polyclonal antibody (26804-1-AP)

    IHC staining of human stomach cancer using 26804-1-AP (same clone as 26804-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26804-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SLC25A12 Polyclonal antibody (26804-1-AP)

    IHC staining of human stomach cancer using 26804-1-AP (same clone as 26804-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26804-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SLC25A12 Polyclonal antibody (26804-1-AP)

    IHC staining of mouse kidney using 26804-1-AP (same clone as 26804-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26804-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SLC25A12 Polyclonal antibody (26804-1-AP)

    IHC staining of mouse kidney using 26804-1-AP (same clone as 26804-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26804-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using SLC25A12 Polyclonal antibody (26804-1-AP)

    IF Staining of mouse heart using 26804-1-AP (same clone as 26804-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed tissue using 26804-1-AP (SLC25A12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26804-1-PBS in a different storage buffer formulation.

SLC25A12 Polyclonal antibody, PBS Only

SLC25A12 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 26804-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

AGC1, Araceli hiperlarga, Aralar, Aralar1, Electrogenic aspartate/glutamate antiporter SLC25A12, mitochondrial

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

26804-1-PBS targets SLC25A12 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag25150

Product name: Recombinant human SLC25A12 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-64 aa of BC016932

Sequence: MAVKVQTTKRGDPHELRNIFLQYASTEVDGERYMTPEDFVQRYLGLYNDPNSNPKIVQLLAGVA

相同性解析による交差性が予測される生物種
Full Name solute carrier family 25 (mitochondrial carrier, Aralar), member 12
Calculated molecular weight 75 kDa, 63 kDa
Observed molecular weight~70 kDa
GenBank accession numberBC016932
Gene Symbol SLC25A12
Gene ID (NCBI) 8604
RRIDAB_2880641
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO75746
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SLC25A12, also known as ARALAR1, is a key component of the malate-aspartate shuttle, a metabolic system that facilitates the transfer of reducing equivalents (NADH) from the cytoplasm into mitochondria, thereby supporting oxidative phosphorylation and ATP production. SLC25A12 dysfunction results in impaired mitochondrial energy production, which can lead to neuronal damage, developmental delays, and myelination defects (PMID: 20015484).

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