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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC25A11 Polyclonal antibody

SLC25A11 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 12253-1-AP

Synonyms

OGC, OGCP, SLC20A4, SLC25A11

Validation Data Gallery

Applications:
  • WB analysis using 12253-1-AP

    WB analysis using 12253-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12253-1-AP (SLC25A11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 12253-1-AP

    WB analysis of HEK-293 using 12253-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12253-1-AP (SLC25A11 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of human kidney using 12253-1-AP

    WB analysis of human kidney using 12253-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12253-1-AP (SLC25A11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 12253-1-AP

    IP experiment of HEK-293 using 12253-1-AP

    IP result of anti-SLC25A11 (IP:12253-1-AP, 4ug; Detection:12253-1-AP 1:1000) with HEK-293 cells lysate 1560ug.

  • IF Staining of HEK-293 using 12253-1-AP

    IF Staining of HEK-293 using 12253-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 12253-1-AP (SLC25A11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, human kidney tissue, HEK-293 cells, Raji cells
Positive IP detected inHEK-293 cells
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12253-1-AP targets SLC25A11 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC25A11 fusion protein Ag2903 相同性解析による交差性が予測される生物種
Full Name solute carrier family 25 (mitochondrial carrier; oxoglutarate carrier), member 11
Calculated molecular weight 314 aa, 34 kDa
Observed molecular weight 28-34 kDa
GenBank accession numberBC016294
Gene symbol SLC25A11
Gene ID (NCBI) 8402
RRIDAB_2877840
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SLC25A11 antibody 12253-1-APDownload protocol
IF protocol for SLC25A11 antibody 12253-1-APDownload protocol
IP protocol for SLC25A11 antibody 12253-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

Proc Natl Acad Sci U S A

Mitochondrial outer membrane protein FUNDC2 promotes ferroptosis and contributes to doxorubicin-induced cardiomyopathy

Authors - Na Ta
  • KO Validated
View Article
humanWB

Science

Autoregulatory control of mitochondrial glutathione homeostasis

Authors - Yuyang Liu
View Article