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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC24A6 Polyclonal antibody (21430-1-AP)

    WB analysis using 21430-1-AP (same clone as 21430-1-PBS)

    Mouse brain tissue were subjected to SDS PAGE followed by western blot with 21430-1-AP (SLC24A6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21430-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SLC24A6 Polyclonal antibody (21430-1-AP)

    WB analysis using 21430-1-AP (same clone as 21430-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21430-1-AP (SLC24A6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21430-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC24A6 Polyclonal antibody (21430-1-AP)

    IHC staining of mouse brain using 21430-1-AP (same clone as 21430-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21430-1-AP (SLC24A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21430-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC24A6 Polyclonal antibody (21430-1-AP)

    IHC staining of mouse brain using 21430-1-AP (same clone as 21430-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21430-1-AP (SLC24A6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21430-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using SLC24A6 Polyclonal antibody (21430-1-AP)

    IHC staining of mouse heart using 21430-1-AP (same clone as 21430-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21430-1-AP (SLC24A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21430-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using SLC24A6 Polyclonal antibody (21430-1-AP)

    IHC staining of mouse heart using 21430-1-AP (same clone as 21430-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21430-1-AP (SLC24A6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21430-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC24A6 Polyclonal antibody (21430-1-AP)

    IHC staining of mouse brain using 21430-1-AP (same clone as 21430-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21430-1-AP (SLC24A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21430-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC24A6 Polyclonal antibody (21430-1-AP)

    IHC staining of mouse brain using 21430-1-AP (same clone as 21430-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21430-1-AP (SLC24A6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21430-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SLC24A6 Polyclonal antibody, PBS Only

SLC24A6 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 21430-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21430-1-PBS targets SLC24A6 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16034

Product name: Recombinant human SLC24A6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 240-333 aa of BC098360

Sequence: YVVTVILCTWIYQRQRRGSLFCPMPVTPEILSDSEEDRVSSNTNSYDYGDEYRPLFFYQETTAQILVRALNPLDYMKWRRKSAYWKALKVFKLP

相同性解析による交差性が予測される生物種
Full Name solute carrier family 24 (sodium/potassium/calcium exchanger), member 6
Calculated molecular weight 584 aa, 64 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC098360
Gene Symbol SLC24A6
Gene ID (NCBI) 80024
RRIDAB_10858637
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6J4K2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SLC24A6, also named as NCLX, is a kind of mitochondrial sodium/calcium antiporter that mediates sodium-dependent calcium efflux from mitochondrion by mediating the exchange of 3 sodium ions per 1 calcium ion. It has been reported that SLC24A6 plays a key role in attenuating potential ROS production and injury upon cardiac reperfusion. SLC24A6 is associated with mitochondrial calcium homeostasis by mediating mitochondrial calcium extrusion. (PMID: 32728214, 20018762, 28219928)

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