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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

SLC24A5 Polyclonal antibody

SLC24A5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27747-1-AP

Synonyms

JSX, NCKX5, SHEP4, SLC24A5

Validation Data Gallery

Applications:
  • WB analysis using 27747-1-AP

    WB analysis using 27747-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27747-1-AP (SLC24A5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of A375 using 27747-1-AP

    WB analysis of A375 using 27747-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 27747-1-AP (SLC24A5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human thyroid cancer using 27747-1-AP

    IHC staining of human thyroid cancer using 27747-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27747-1-AP (SLC24A5 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human thyroid cancer using 27747-1-AP

    IHC staining of human thyroid cancer using 27747-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27747-1-AP (SLC24A5 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse eye tissue, A375 cells, rat eye tissue
Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

27747-1-AP targets SLC24A5 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC24A5 fusion protein Ag24254 相同性解析による交差性が予測される生物種
Full Name solute carrier family 24, member 5
Calculated molecular weight 500 aa, 55 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC113628
Gene symbol SLC24A5
Gene ID (NCBI) 283652
RRIDAB_2880959
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SLC24A5 antibody 27747-1-APDownload protocol
IHC protocol for SLC24A5 antibody 27747-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Lab Invest

Nuclear receptor binding factor 2 (NRBF2) is required for learning and memory.

Authors - Xiaosen Ouyang
View Article
mouseIHC

Pigment Cell Melanoma Res

Impact of a SLC24A5 variant on the retinal pigment epithelium of a Japanese patient with oculocutaneous albinism type 6.

Authors - Toru Saito
  • KO Validated
View Article