Validation Data Gallery

Filter: AllWBIHCIF-P

WB analysis using 18992-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18992-1-AP (SLC24A4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa cells using 18992-1-AP
WB result of SLC24A4 antibody (18992-1-AP, 1:1000) with si-Control and si-SLC24A4 transfected HeLa cells.
WB analysis of mouse brain using 18992-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 18992-1-AP (SLC24A4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 18992-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 18992-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 18992-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 18992-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 18992-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 18992-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse eye using 18992-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using SLC24A4 antibody (18992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse eye using 18992-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using SLC24A4 antibody (18992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

SLC24A4 Polyclonal antibody

SLC24A4 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 18992-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF-P, ELISA

Synonyms

Solute carrier family 24 member 4, Sodium/potassium/calcium exchanger 4, NCKX4, Na(+)/K(+)/Ca(2+)-exchange protein 4

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, HeLa cells, mouse heart tissue
Positive IHC detected in	mouse brain tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse eye tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IHC	See 2 publications below
IF	See 1 publications below

Product Information

18992-1-AP targets SLC24A4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	solute carrier family 24 (sodium/potassium/calcium exchanger), member 4
Calculated molecular weight	69 kDa
Observed molecular weight	62-90 kDa
GenBank accession number	NM_153646
Gene Symbol	SLC24A4
Gene ID (NCBI)	123041
RRID	AB_10596939
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8NFF2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SLC24A4, also named as NCKX4, belongs to the sodium/potassium/calcium exchanger family. SLC24A4 transports 1 Ca2+ and 1 K+ in exchange for 4 Na+. Genetic variations in SLC24A4 are associated with skin/hair/eye pigmentation type 6 (SHEP6).

Protocols

Product Specific Protocols
IF protocol for SLC24A4 antibody 18992-1-AP	Download protocol
IHC protocol for SLC24A4 antibody 18992-1-AP	Download protocol
WB protocol for SLC24A4 antibody 18992-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	Bone Deletion of epithelial cell-specific p130Cas impairs the maturation stage of amelogenesis. Authors - Akane Inoue View Article
rat	WB	J Cell Physiol Identification of novel candidate genes involved in mineralization of dental enamel by genome-wide transcript profiling. Authors - Lacruz Rodrigo S RS View Article
Mouse	IHC,IF	J Dent Res Ameloblast Modulation and Transport of Cl-, Na+, and K+ during Amelogenesis. Authors - A L J J Bronckers View Article
mouse	WB	Calcif Tissue Int Reduced Protein Expression of the Na(+)/Ca(2+)+K(+)-Exchanger (SLC24A4) in Apical Plasma Membranes of Maturation Ameloblasts of Fluorotic Mice. Authors - A L J J Bronckers View Article
human	WB	PeerJ Identification and validation of calcium extrusion-related genes prognostic signature in colon adenocarcinoma Authors - Mingpeng Jin View Article
mouse	WB	Cells Tissues Organs Expression of the sodium/calcium/potassium exchanger, NCKX4, in ameloblasts. Authors - Hu Ping P View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 18992-1-AP

WB analysis of HeLa cells using 18992-1-AP

WB analysis of mouse brain using 18992-1-AP

IHC staining of mouse brain using 18992-1-AP

IHC staining of mouse brain using 18992-1-AP

IHC staining of mouse brain using 18992-1-AP

IHC staining of mouse brain using 18992-1-AP

IHC staining of human heart using 18992-1-AP

IHC staining of human heart using 18992-1-AP

IF Staining of mouse eye using 18992-1-AP

IF Staining of mouse eye using 18992-1-AP

SLC24A4 Polyclonal antibody

SLC24A4 Polyclonal Antibody for WB, IHC, IF-P, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Deletion of epithelial cell-specific p130Cas impairs the maturation stage of amelogenesis.

Identification of novel candidate genes involved in mineralization of dental enamel by genome-wide transcript profiling.

Ameloblast Modulation and Transport of Cl-, Na+, and K+ during Amelogenesis.

Reduced Protein Expression of the Na(+)/Ca(2+)+K(+)-Exchanger (SLC24A4) in Apical Plasma Membranes of Maturation Ameloblasts of Fluorotic Mice.

Identification and validation of calcium extrusion-related genes prognostic signature in colon adenocarcinoma

Expression of the sodium/calcium/potassium exchanger, NCKX4, in ameloblasts.