Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293T cells, PC-12 cells, mouse kidney tissue, mouse liver tissue, rat kidney tissue, rat liver tissue |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31069-1-AP targets SLC22A10 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag34521 Product name: Recombinant human SLC22A10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 281-349 aa of NM_001039752 Sequence: ESARWLIITNKLDEGLKALRKVARTNGIKNAEETLNIEVVRSTMQEELDAAQTKTTVCDLFRNPSMRKR 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 22, member 10 |
| Calculated molecular weight | 541 aa, 60 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | NM_001039752 |
| Gene Symbol | SLC22A10 |
| Gene ID (NCBI) | 387775 |
| RRID | AB_3669842 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q63ZE4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Organic anion transporter 5 (OAT5), also known as SLC22A10, belongs to the solute carrier group of membrane transport proteins that mediate cellular uptake of endogenous and exogenous organic ions (PMID: 20865662). Rare variants in SLC22A10 may be associated with Alzheimer's disease CSF profile of neuronal injury and inflammation (PMID: 35173266).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SLC22A10 antibody 31069-1-AP | Download protocol |
| WB protocol for SLC22A10 antibody 31069-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |