Validation Data Gallery

Filter: AllWBIPIHCIF-P

WB analysis using 12820-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12820-1-AP (SLC20A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 12820-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 12820-1-AP (SLC20A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of COLO 320 using 12820-1-AP
IP result of anti-SLC20A2 (IP:12820-1-AP, 4ug; Detection:12820-1-AP 1:400) with COLO 320 cells lysate 2400ug.
IHC staining of mouse testis using 12820-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12820-1-AP (SLC20A2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 12820-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12820-1-AP (SLC20A2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human bowen disease using 12820-1-AP
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 12820-1-AP (SLC20A2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human bowen disease using 12820-1-AP
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 12820-1-AP (SLC20A2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 12820-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SLC20A2 antibody (12820-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

SLC20A2 Polyclonal antibody

SLC20A2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 12820-1-AP

Publications(14)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF-P, IP, ELISA

Synonyms

Gibbon ape leukemia virus receptor 2, GLVR2, GLVR-2, hPit2, Phosphate transporter 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (14)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, COLO 320 cells, mouse kidney tissue
Positive IP detected in	COLO 320 cells
Positive IHC detected in	human bowen disease, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse brain tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 12 publications below
IF	See 2 publications below

Product Information

12820-1-AP targets SLC20A2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3469 Product name: Recombinant human SLC20A2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 231-512 aa of BC028600 Sequence: WLFVCPWMRRKITGKLQKEGALSRVSDESLSKVQEAESPVFKELPGAKANDDSTIPLTGAAGETLGTSEGTSAGSHPRAAYGRALSMTHGSVKSPISNGTFGFDGHTRSDGHVYHTVHKDSGLYKDLLHKIHIDRGPEEKPAQESNYRLLRRNNSYTCYTAAICGLPVHATFRAADSSAPEDSEKLVGDTVSYSKKRLRYDSYSSYCNAVAEAEIEAEEGGVEMKLASELADPDQPREDPAEEEKEEKDAPEVHLLFHFLQVLTACFGSFAHGGNDVSNAIG 相同性解析による交差性が予測される生物種
Full Name	solute carrier family 20 (phosphate transporter), member 2
Calculated molecular weight	652 aa, 70 kDa
Observed molecular weight	68-70 kDa
GenBank accession number	BC028600
Gene Symbol	SLC20A2
Gene ID (NCBI)	6575
RRID	AB_2191004
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q08357
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SLC20A2 (also known as PiT2) is a sodium (Na)-phosphate (Pi) co-transporter that belongs to the SLC20 family. SLC20A2 is highly expressed in the central nervous system and controls hippocampal-dependent learning and memory (PMID: 38195526). SLC20A2 is the major causative gene for primary familial brain calcification (PFBC), a rare neurological disease characterized by widespread cognitive impairment.

Protocols

Product Specific Protocols
IF protocol for SLC20A2 antibody 12820-1-AP	Download protocol
IHC protocol for SLC20A2 antibody 12820-1-AP	Download protocol
IP protocol for SLC20A2 antibody 12820-1-AP	Download protocol
WB protocol for SLC20A2 antibody 12820-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Cell Death Dis Slc20a1 and Slc20a2 regulate neuronal plasticity and cognition independently of their phosphate transport ability Authors - Mariana Ramos-Brossier View Article
human	WB	Adv Healthc Mater Modulating Temporospatial Phosphate Equilibrium by Nanoparticulate Mineralized Collagen Materials Induces Osteogenesis via PiT-1 and PiT-2 Authors - Xiaoyan Ren View Article
human	WB	Adv Healthc Mater Correlating Material Properties to Osteoprotegerin Expression on Nanoparticulate Mineralized Collagen Glycosaminoglycan Scaffolds Authors - Wei Chen KD Validated View Article
human	WB	Arterioscler Thromb Vasc Biol Potential Role of H-Ferritin in Mitigating Valvular Mineralization. Authors - Katalin Éva Sikura View Article
human	WB	Mov Disord A Homozygous Variant in NAA60 Is Associated with Primary Familial Brain Calcification Authors - Xinhui Chen View Article
human	WB	Cell Rep Homeostatic coordination of cellular phosphate uptake and efflux requires an organelle-based receptor for the inositol pyrophosphate IP8 Authors - Xingyao Li View Article

製品

アプリケーション

cGMPグレード製品

サポート

カスタム対応

プロモーション

企業情報

抗体

免疫アッセイ

サイトカイン・増殖因子

ChromoTek VHH（Nanobody®）試薬

サポートツール・試薬

ゲノミクス試薬

研究分野

Validation Data Gallery

WB analysis using 12820-1-AP

WB analysis of COLO 320 using 12820-1-AP

IP experiment of COLO 320 using 12820-1-AP

IHC staining of mouse testis using 12820-1-AP

IHC staining of mouse testis using 12820-1-AP

IHC staining of human bowen disease using 12820-1-AP

IHC staining of human bowen disease using 12820-1-AP

IF Staining of mouse brain using 12820-1-AP

SLC20A2 Polyclonal antibody

SLC20A2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Slc20a1 and Slc20a2 regulate neuronal plasticity and cognition independently of their phosphate transport ability

Modulating Temporospatial Phosphate Equilibrium by Nanoparticulate Mineralized Collagen Materials Induces Osteogenesis via PiT-1 and PiT-2

Correlating Material Properties to Osteoprotegerin Expression on Nanoparticulate Mineralized Collagen Glycosaminoglycan Scaffolds

Potential Role of H-Ferritin in Mitigating Valvular Mineralization.

A Homozygous Variant in NAA60 Is Associated with Primary Familial Brain Calcification

Homeostatic coordination of cellular phosphate uptake and efflux requires an organelle-based receptor for the inositol pyrophosphate IP8

Able^™

Validation Data Gallery

WB analysis using 12820-1-AP

WB analysis of COLO 320 using 12820-1-AP

IP experiment of COLO 320 using 12820-1-AP

IHC staining of mouse testis using 12820-1-AP

IHC staining of mouse testis using 12820-1-AP

IHC staining of human bowen disease using 12820-1-AP

IHC staining of human bowen disease using 12820-1-AP

IF Staining of mouse brain using 12820-1-AP

SLC20A2 Polyclonal antibody

SLC20A2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Slc20a1 and Slc20a2 regulate neuronal plasticity and cognition independently of their phosphate transport ability

Modulating Temporospatial Phosphate Equilibrium by Nanoparticulate Mineralized Collagen Materials Induces Osteogenesis via PiT-1 and PiT-2

Correlating Material Properties to Osteoprotegerin Expression on Nanoparticulate Mineralized Collagen Glycosaminoglycan Scaffolds

Potential Role of H-Ferritin in Mitigating Valvular Mineralization.

A Homozygous Variant in NAA60 Is Associated with Primary Familial Brain Calcification

Homeostatic coordination of cellular phosphate uptake and efflux requires an organelle-based receptor for the inositol pyrophosphate IP8

Able™

Able^™