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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using SLC18A1 Polyclonal antibody (20340-1-AP)

    WB analysis of HEK-293 using 20340-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20340-1-AP (SLC18A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using SLC18A1 Polyclonal antibody (20340-1-AP)

    WB analysis of A549 using 20340-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 20340-1-AP (SLC18A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C6 cells using SLC18A1 Polyclonal antibody (20340-1-AP)

    WB analysis of C6 using 20340-1-AP

    C6 cells were subjected to SDS PAGE followed by western blot with 20340-1-AP (SLC18A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SLC18A1 Polyclonal antibody (20340-1-AP)

    WB analysis of HeLa using 20340-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20340-1-AP (SLC18A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SLC18A1 Polyclonal antibody (20340-1-AP)

    WB analysis of HepG2 using 20340-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20340-1-AP (SLC18A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using SLC18A1 Polyclonal antibody (20340-1-AP)

    IHC staining of human placenta using 20340-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 20340-1-AP (SLC18A1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using SLC18A1 Polyclonal antibody (20340-1-AP)

    IHC staining of human placenta using 20340-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 20340-1-AP (SLC18A1 antibody) at dilution of 1:100 (under 40x lens).

SLC18A1 Polyclonal antibody

SLC18A1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20340-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Synonyms

CGAT, SLC18A1, VAT1, Vesicular amine transporter 1, VMAT1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, A549 cells, C6 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20340-1-AP targets SLC18A1 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14131

Product name: Recombinant human SLC18A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 43-138 aa of BC009387

Sequence: PIVPTFLYDMEFKEVNSSLHLGHAGSSPHALASPAFSTIFSFFNNNTVAVEESVPSGIAWMNDTASTIPPPATEAISAHKNNCLQGTGFLEEEITR

相同性解析による交差性が予測される生物種
Full Name solute carrier family 18 (vesicular monoamine), member 1
Calculated molecular weight 525 aa, 56 kDa
Observed molecular weight 50 kDa, 56 kDa
GenBank accession numberBC009387
Gene Symbol SLC18A1
Gene ID (NCBI) 6570
RRIDAB_10666128
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP54219
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for SLC18A1 antibody 20340-1-APDownload protocol
WB protocol for SLC18A1 antibody 20340-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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