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Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using SLC17A5 Polyclonal antibody (13320-1-AP)

    WB analysis of human kidney using 13320-1-AP (same clone as 13320-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 13320-1-AP (SLC17A5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13320-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using SLC17A5 Polyclonal antibody (13320-1-AP)

    WB analysis of HEK-293 using 13320-1-AP (same clone as 13320-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13320-1-AP (SLC17A5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13320-1-PBS in a different storage buffer formulation.

SLC17A5 Polyclonal antibody, PBS Only

SLC17A5 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 13320-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Sialin, AST, H(+)/nitrate cotransporter, H(+)/sialic acid cotransporter, ISSD

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13320-1-PBS targets SLC17A5 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3855

Product name: Recombinant human SLC17A5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 166-496 aa of BC020961

Sequence: VLRALEGLGEGVTFPAMHAMWSSWAPPLERSKLLSISYAGAQLGTVISLPLSGIICYYMNWTYVFYFFGTIGIFWFLLWIWLVSDTPQKHKRISHYEKEYILSSLRNQLSSQKSVPWVPILKSLPLWAIVVAHFSYNWTFYTLLTLLPTYMKEILRFNVQENGFLSSLPYLGSWLCMILSGQAADNLRAKWNFSTLCVRRIFSLIGMIGPAVFLVAAGFIGCDYSLAVAFLTISTTLGGFCSSGFSINHLDIAPSYAGILLGITNTFATIPGMVGPVIAKSLTPDNTVGEWQTVFYIAAAINVFGAIFFTLFAKGEVQNWALNDHHGHRH

相同性解析による交差性が予測される生物種
Full Name solute carrier family 17 (anion/sugar transporter), member 5
Calculated molecular weight 495 aa, 55 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC020961
Gene Symbol SLC17A5
Gene ID (NCBI) 26503
RRIDAB_2187524
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRA2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SLC17A5 encodes a transmembrane protein, sialin, which transports free sialic acid across the lysosomal membrane. Mutations in sialin result in neurodegenerative sialic acid storage disorders, Salla disease (SD) and infantile sialic acid storage disease (ISSD). In the central nervous system, sialin is more abundant in neurons than in glia. It has been reported to be localized into lysosome in neuron but in plasma membrane in nonneuronal cells (PMID: 15006695).

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