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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using SLC16A5 Polyclonal antibody (12120-1-AP)

    WB analysis of mouse skeletal muscle using 12120-1-AP (same clone as 12120-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12120-1-AP (SLC16A5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12120-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using SLC16A5 Polyclonal antibody (12120-1-AP)

    WB analysis of human placenta using 12120-1-AP (same clone as 12120-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 12120-1-AP (SLC16A5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12120-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SLC16A5 Polyclonal antibody (12120-1-AP)

    IHC staining of human breast cancer using 12120-1-AP (same clone as 12120-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12120-1-AP (SLC16A5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12120-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SLC16A5 Polyclonal antibody (12120-1-AP)

    IHC staining of human kidney using 12120-1-AP (same clone as 12120-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12120-1-AP (SLC16A5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12120-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SLC16A5 Polyclonal antibody (12120-1-AP)

    IHC staining of human kidney using 12120-1-AP (same clone as 12120-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12120-1-AP (SLC16A5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12120-1-PBS in a different storage buffer formulation.

SLC16A5 Polyclonal antibody, PBS Only

SLC16A5 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12120-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12120-1-PBS targets SLC16A5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2762

Product name: Recombinant human SLC16A5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 418-505 aa of BC009684

Sequence: ALQKKEQGKQAVAADALERDLFLEAKDGPGKQRSPEIMCQSSRQPRPAGVNKHLWGCPASSRTSHEWLLWPKAVLQAKQTALGWNSPT

相同性解析による交差性が予測される生物種
Full Name solute carrier family 16, member 5 (monocarboxylic acid transporter 6)
Calculated molecular weight 505 aa, 55 kDa
Observed molecular weight50-55 kDa
GenBank accession numberBC009684
Gene Symbol SLC16A5
Gene ID (NCBI) 9121
RRIDAB_2877827
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO15375
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Monocarboxylate transporter 5 (SLC16A5), also named as MCT6, is a member of the MCT family. SLC16A5 is a proton-linked monocarboxylate transporter that catalyzes the rapid transport across the plasma membrane of many monocarboxylates and it can transport various drugs. It has been reported that SLC16A5 mutation may be a novel genetic risk factor for cisplatin-induced ototoxic (CIO) in testicular cancer patients. There are some isoforms of SLC16A5 among 50-55 kDa. (PMID: 16174808, 28448657)

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