Validation Data Gallery
Tested Applications
| Positive WB detected in | COLO 320 cells, Caco-2 cells, HT-29 cells, MOLT-4 cells, mouse small intestine tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 1 publications below |
Product Information
21726-1-AP targets SLC15A1/PEPT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16390 Product name: Recombinant human SLC15A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 374-580 aa of BC096327 Sequence: VAAIVQVEIDKTLPVFPKGNEVQIKVLNIGNNTMNISLPGEMVTLGPMSQTNAFMTFDVNKLTRINISSPGSPVTAVTDDFKQGQRHTLLVWAPNHYQVVKDGLNQKPEKGENGIRFVNTFNELITITMSGKVYANISSYNASTYQFFPSGIKGFTISSTEIPPQCQPNFNTFYLEFGSAYTYIVQRKNDSCPEVKVFEDISANTVN 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 15 (oligopeptide transporter), member 1 |
| Calculated molecular weight | 708 aa, 79 kDa |
| Observed molecular weight | 70-80 kDa |
| GenBank accession number | BC096327 |
| Gene Symbol | SLC15A1/PEPT1 |
| Gene ID (NCBI) | 6564 |
| RRID | AB_3085668 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P46059 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SLC15A1/PEPT1 is a peptide transporter that transports oligopeptides of 2 to 4 amino acids with a preference for dipeptides. PEPT1 mediates transepithelial transport of muramyl and N-formylated bacterial dipeptides contributing to recognition of pathogenic bacteria by the mucosal immune system. PEPT1 is primarily expressed in the small intestine (PMID: 22194420). PEPT1 is also expressed in distal colon in rodents and humans and contributes to water absorption. 71 kDa nonglycosylated PEPT1 protein can be detected (PMID: 23660505).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC15A1/PEPT1 antibody 21726-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Engineered vesicles enhance oral antibiotic absorption in proximal small intestine and mitigate gut dysbiosis. | ||
Diabetes Obes Metab Rapid improvement of renal microcirculatory homeostasis by liraglutide following diabetes induction. | ||
J Am Heart Assoc Stanniocalcin-1 Attenuates Cardiac Fibrosis Post Ischemia-Reperfusion Injury by Targeting the DOCKER Domain of Dedicator of Cytokinesis 1. | ||
Photodiagnosis Photodyn Ther Expression of coproporphyrinogen oxidase is associated with detection of upper gastrointestinal carcinomas by 5-aminolevulinic acid-mediated photodynamic diagnosis. | ||
J Oral Biosci RANKL enhances the expression of PEPT1/SLC15A1 and PEPT2/SLC15A2 in RAW264.7 cells. |