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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using SLC13A4 Polyclonal antibody (20302-1-AP)

    WB analysis of HEK-293 using 20302-1-AP (same clone as 20302-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20302-1-AP (SLC13A4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20302-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using SLC13A4 Polyclonal antibody (20302-1-AP)

    WB analysis of HEK-293 using 20302-1-AP (same clone as 20302-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20302-1-AP (SLC13A4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20302-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SLC13A4 Polyclonal antibody (20302-1-AP)

    IF Staining of HEK-293 using 20302-1-AP (same clone as 20302-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 20302-1-AP (SLC13A4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20302-1-PBS in a different storage buffer formulation.

SLC13A4 Polyclonal antibody, PBS Only

SLC13A4 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 20302-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20302-1-PBS targets SLC13A4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14116

Product name: Recombinant human SLC13A4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 123-285 aa of BC030689

Sequence: LLLCFMCCTTLLSMWLSNTSTTAMVMPIVEAVLQELVSAEDEQLVAGNSNTEEAEPISLDVKNSQPSLELIFVNEESNADLTTLMHNENLNGVPSITNPIKTANQHQGKKQHPSQEKPQVLTRSPRKQKLNRKYRSHHDQMICKCLSLSISYSATIGGLTTII

相同性解析による交差性が予測される生物種
Full Name solute carrier family 13 (sodium/sulfate symporters), member 4
Calculated molecular weight 626 aa, 69 kDa
Observed molecular weight 69 kDa
GenBank accession numberBC030689
Gene Symbol SLC13A4
Gene ID (NCBI) 26266
RRIDAB_10793722
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKG4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SLC13A4 (also known as SUT-1 or NaS2) belongs to the family of Na+-coupled anion transporters and mediates sulfate reabsorption in the high endothelial venules (HEV). Sulfate is an obligate nutrient for fetal development. Highly expressed in placenta, SLC13A4 has been proposed to play important physiological roles in maintaining high maternal serum sulfate levels during pregnancy and mediating sulfate supply to the fetus.

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