Validation Data Gallery
Tested Applications
| Positive WB detected in | 37°C incubated mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12242-1-AP targets SLC12A8 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2886 Product name: Recombinant human SLC12A8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 409-714 aa of BC020506 Sequence: SCSLTPVPEPVLREGAEGLHCSEHLLLEKAPSYGSEGPAQRVLEGTLLEFTKDMDQLLQLTRKLESSQPRQGEGNRTPESQKRKSKKATKQTLQDSFLLDLKSPPSFPVEISDRLPAASWEGQESCWNKQTSKSEGTQPEGTYGEQLVPELCNQSESSGEDFFLKSRLQEQDVWRRSTSFYTHMCNPWVSLLGAVGSLLIMFVIQWVYTLVNMGVAAIVYFYIGRASPGLHLGSASNFSFFRWMRSLLLPSCRSLQSPQEQIILAPSLAKVDMEMTQLTQENADFATRDRYHHSSLVNREQLMPHY 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 12 (potassium/chloride transporters), member 8 |
| Calculated molecular weight | 714 aa, 80 kDa |
| Observed molecular weight | 70 KDa |
| GenBank accession number | BC020506 |
| Gene Symbol | SLC12A8 |
| Gene ID (NCBI) | 84561 |
| RRID | AB_3669135 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | A0AV02 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Solute carrier family 12 member 8 (SLC12A8) belongs to the SLC12 gene family, which consists of electroneutral cation-chloride coupled cotransporters. It is highly expressed in small intestine and pancreas and moderately expressed in liver and white adipose tissue (PMID: 31131364). SLC12A8 may be a useful biomarker for the early diagnosis and treatment of bladder cancer (PMID: 33364434).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC12A8 antibody 12242-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |