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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SLC12A4/KCC1 Polyclonal antibody (15927-1-AP)

    WB analysis of HeLa using 15927-1-AP (same clone as 15927-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15927-1-AP (SLC12A4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15927-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SLC12A4/KCC1 Polyclonal antibody (15927-1-AP)

    WB analysis of HeLa using 15927-1-AP (same clone as 15927-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15927-1-AP (SLC12A4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15927-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using SLC12A4/KCC1 Polyclonal antibody (15927-1-AP)

    IP experiment of HeLa using 15927-1-AP (same clone as 15927-1-PBS)

    IP result of anti-SLC12A4 (IP:15927-1-AP, 4ug; Detection:15927-1-AP 1:500) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 15927-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLC12A4/KCC1 Polyclonal antibody (15927-1-AP)

    IHC staining of human liver cancer using 15927-1-AP (same clone as 15927-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15927-1-AP (SLC12A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15927-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLC12A4/KCC1 Polyclonal antibody (15927-1-AP)

    IHC staining of human liver cancer using 15927-1-AP (same clone as 15927-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15927-1-AP (SLC12A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15927-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLC12A4/KCC1 Polyclonal antibody (15927-1-AP)

    IHC staining of human liver cancer using 15927-1-AP (same clone as 15927-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15927-1-AP (SLC12A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15927-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLC12A4/KCC1 Polyclonal antibody (15927-1-AP)

    IHC staining of human liver cancer using 15927-1-AP (same clone as 15927-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15927-1-AP (SLC12A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15927-1-PBS in a different storage buffer formulation.

SLC12A4/KCC1 Polyclonal antibody, PBS Only

SLC12A4/KCC1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 15927-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

SLC12A4, Electroneutral potassium-chloride cotransporter 1, Erythroid K-Cl cotransporter 1, KCC1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15927-1-PBS targets SLC12A4/KCC1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8762

Product name: Recombinant human SLC12A4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 891-1085 aa of BC021193

Sequence: MKKDLAVFLYHLRLEAEVEVVEMHNSDISAYTYERTLMMEQRSQMLRQMRLTKTEREREAQLVKDRHSALRLESLYSDEEDESAVGADKIQMTWTRDKYMTETWDPSHAPDNFRELVHIKPDQSNVRRMHTAVKLNEVIVTRSHDARLVLLNMPGPPRNSEGDENYMEFLEVLTEGLERVLLVRGGGREVITIYS

相同性解析による交差性が予測される生物種
Full Name solute carrier family 12 (potassium/chloride transporters), member 4
Calculated molecular weight 1085 aa, 121 kDa
Observed molecular weight 121 kDa
GenBank accession numberBC021193
Gene Symbol SLC12A4
Gene ID (NCBI) 6560
RRIDAB_2188666
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UP95
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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