Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13636-1-AP targets SLC12A1/NKCC2 in WB, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4564 Product name: Recombinant human SLC12A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-176 aa of BC040138 Sequence: MSLNNSSNVFLDSVPSNTNRFQVSVINENHESSAAADDNTDPPHYEETSFGDEAQKRLRISFRPGNQECYDNFLQSGETAKTDASFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSISGPKVNRPSLLEIHEQLAKNVAVTPSSADRVANGDGIPGDEQAENKEDDQAGVVK 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 12 (sodium/potassium/chloride transporters), member 1 |
| Calculated molecular weight | 121 kDa |
| Observed molecular weight | 140-180 kDa |
| GenBank accession number | BC040138 |
| Gene Symbol | SLC12A1/NKCC2 |
| Gene ID (NCBI) | 6557 |
| RRID | AB_3085420 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13621 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC12A1/NKCC2 antibody 13636-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
