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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLAMF3/CD229 Polyclonal antibody (31717-1-AP)

    WB analysis using 31717-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31717-1-AP (SLAMF3/CD229 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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CD229/SLAMF3 Polyclonal antibody

CD229/SLAMF3 Polyclonal Antibody for WB, ELISA
Cat No. 31717-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

SLAMF3, hly9, Cell surface molecule Ly-9, Cell surface molecule Ly 9, CDABP0070

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU266 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31717-1-AP targets CD229/SLAMF3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD229/SLAMF3 fusion protein Ag34617 相同性解析による交差性が予測される生物種
Full Name lymphocyte antigen 9
Observed molecular weight90 kDa, 110 kDa
GenBank accession numberBC121049
Gene Symbol LY9
Gene ID (NCBI) 4063
RRIDAB_3670094
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HBG7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Signaling lymphocytic activation molecule 3 (SLAMF3), also known as CD229 or Ly9, is a type I transmembrane glycoprotein that belongs to the SLAM family receptors (PMID: 10970093; 15905546). It is composed of an extracellular region, which consists of four Ig-like domains, a transmembrane region, and a cytoplasmic tail, which includes two immunoreceptor tyrosine-based signaling motifs (ITSMs) and other tyrosine residues that act as binding sites for the SH2 structural domains (PMID: 8537117; 11369645; 38626547). SLAMF3 is mainly expressed in immune cells, such as T, B, and natural killer cells, and it is involved in the fundamental activation and suppression of these immune cells, as well as the regulation of complex immune networks (PMID: 38626547).

Protocols

Product Specific Protocols
WB protocol for CD229/SLAMF3 antibody 31717-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols