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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SKP2 Recombinant antibody (85670-4-RR)

    WB analysis using 85670-4-RR (same clone as 85670-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85670-4-RR (SKP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85670-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SKP2 Recombinant antibody (85670-4-RR)

    IF Staining of HepG2 using 85670-4-RR (same clone as 85670-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SKP2 antibody (85670-4-RR, Clone: 243080H6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85670-4-PBS in a different storage buffer formulation.

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SKP2 Recombinant antibody, PBS Only

SKP2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85670-4-PBS
Clone No.243080H6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Cyclin-A/CDK2-associated protein p45, F box protein Skp2, F box/LRR repeat protein 1, FBL1, F-box protein Skp2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85670-4-PBS targets SKP2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SKP2 fusion protein Ag6826 相同性解析による交差性が予測される生物種
Full Name S-phase kinase-associated protein 2 (p45)
Calculated molecular weight 48 kDa
Observed molecular weight48 kDa, 46 kDa
GenBank accession numberBC001441
Gene Symbol SKP2
Gene ID (NCBI) 6502
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13309
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SKP2(S-phase kinase-associated protein 2) is also named as FBXL1 and belongs to the F-box family. It regulates cellular proliferation by targeting several cell cycle-regulated proteins for ubiquitination and degradation, including cyclin-dependent kinase inhibitor CDKN1B(PMID:22200179). The acetylation of SKP2 in the nuclear localization signal (NLS) can promote its cytoplasmic retention, and cytoplasmic SKP2 enhances cellular migration through ubiquitination and destruction of CDH. This protein has 3 isoforms produced by alternative splicing (PMID:26497688, PMID:16902410).