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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SKP2 Recombinant monoclonal antibody (85670-4-RR)

    WB analysis using 85670-4-RR (same clone as 85670-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85670-4-RR (SKP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85670-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using SKP2 Recombinant monoclonal antibody (85670-4-RR)

    IHC staining of human placenta using 85670-4-RR (same clone as 85670-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85670-4-RR (SKP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85670-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SKP2 Recombinant monoclonal antibody (85670-4-RR)

    IF Staining of HepG2 using 85670-4-RR (same clone as 85670-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SKP2 antibody (85670-4-RR, Clone: 243080H6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85670-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85670-4-RR

    Affinity and Kinetic Characterization of 85670-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85670-4-RR against Human SKP2 were performed. The affinity constant is 0.317 nM.

SKP2 Recombinant monoclonal antibody, PBS Only

SKP2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 85670-4-PBS
Clone No.243080H6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Cyclin-A/CDK2-associated protein p45, F box protein Skp2, F box/LRR repeat protein 1, FBL1, F-box protein Skp2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85670-4-PBS targets SKP2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag6826

Product name: Recombinant human SKP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-344 aa of BC001441

Sequence: MHRKHLQEIPDLSSNVATSFTWGWDSSKTSELLSGMGVSALEKEEPDSENIPQELLSNLGHPESPPRKRLKSKGSDKDFVIVRRPKLNRENFPGVSWDSLPDELLLGIFSCLCLPELLKVSGVCKRWYRLASDESLWQTLDLTGKNLHPDVTGRLLSQGVIAFRCPRSFMDQPLAEHFSPFRVQHMDLSNSVIEVSTLHGILSQCSKLQNLSLEGLRLSDPIVNTLAKNSNLVRLNLSGCSGFSEFALQTLLSSCSRLDELNLSWCFDFTEKHVQVAVAHVSETITQLNLSGYRKNLQKSDLSTLVRRCPNLVHLDLSDSVMLKNDCFQEFFQLNYLQHLSLSR

相同性解析による交差性が予測される生物種
Full Name S-phase kinase-associated protein 2 (p45)
Calculated molecular weight 48 kDa
Observed molecular weight48 kDa, 46 kDa
GenBank accession numberBC001441
Gene Symbol SKP2
Gene ID (NCBI) 6502
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ13309
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SKP2(S-phase kinase-associated protein 2) is also named as FBXL1 and belongs to the F-box family. It regulates cellular proliferation by targeting several cell cycle-regulated proteins for ubiquitination and degradation, including cyclin-dependent kinase inhibitor CDKN1B(PMID:22200179). The acetylation of SKP2 in the nuclear localization signal (NLS) can promote its cytoplasmic retention, and cytoplasmic SKP2 enhances cellular migration through ubiquitination and destruction of CDH. This protein has 3 isoforms produced by alternative splicing (PMID:26497688, PMID:16902410).

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