Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, mouse cerebellum tissue, rat brain tissue |
| Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse cerebellum tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55370-1-AP targets SKOR2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | functional smad suppressing element 18 |
| Calculated molecular weight | 104 kDa |
| Observed molecular weight | 30kDa, 60 kDa |
| GenBank accession number | XM_001715219 |
| Gene Symbol | SKOR2 |
| Gene ID (NCBI) | 652991 |
| RRID | AB_3086446 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q2VWA4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SKOR2 (SKI family transcriptional corepressor 2), also named FUSSEL18, is a transcriptional repressor that is highly expressed in the central nervous system (PMID: 16200078). SKOR2 was detected at 30 and 60 kD in the cerebellum. It is identified as a novel transcriptional regulator in Purkinje cells that acts upstream of Shh during cerebellum development (PMID: 21937600). Skor2 was detected in Purkinje cells in the human fetal cerebellum (PMID: 34140698).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SKOR2 antibody 55370-1-AP | Download protocol |
| IHC protocol for SKOR2 antibody 55370-1-AP | Download protocol |
| WB protocol for SKOR2 antibody 55370-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |