Validation Data Gallery

Filter: AllWBIPIHCIF-P

WB analysis of HepG2 using 12719-2-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12719-2-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 12719-2-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 12719-2-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 12719-2-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 12719-2-AP
IP result of anti-SKIV2L2 (IP:12719-2-AP, 4ug; Detection:12719-2-AP 1:6000) with HepG2 cells lysate 1320 ug.
IHC staining of human stomach using 12719-2-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12719-2-AP (SKIV2L2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse testis using 12719-2-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using SKIV2L2 antibody (12719-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SKIV2L2 Polyclonal antibody

SKIV2L2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 12719-2-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Synonyms

Exosome RNA helicase MTR4, EC:3.6.4.13, DOB1, ATP-dependent RNA helicase SKIV2L2, ATP-dependent RNA helicase DOB1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, human brain tissue, mouse testis tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse testis tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IHC	See 1 publications below
IF	See 1 publications below
IP	See 2 publications below

Product Information

12719-2-AP targets SKIV2L2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3414 Product name: Recombinant human SKIV2L2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 3-307 aa of BC028604 Sequence: DAFGDELFSVFEGDSTTAAGTKKDKEKDKGKWKGPPGSADKAGKRFDGKLQSESTNNGKNKRDVDFEGTDEPIFGKKPRIEESITEDLSLADLMPRVKVQSVETVEGCTHEVALPAEEDYLPLKPRVGKAAKEYPFILDAFQREAIQCVDNNQSVLVSAHTSAGKTVCAEYAIALALREKQRVIFTSPIKALSNQKYREMYEEFQDVGLMTGDVTINPTASCLVMTTEILRSMLYRGSEVMREVAWVIFDEIHYMRDSERGVVWEETIILLPDNVHYVFLSATIPNARQFAEWICHLHKQPCHVI 相同性解析による交差性が予測される生物種
Full Name	superkiller viralicidic activity 2-like 2 (S. cerevisiae)
Calculated molecular weight	1042 aa, 118 kDa
Observed molecular weight	118 kDa
GenBank accession number	BC028604
Gene Symbol	SKIV2L2
Gene ID (NCBI)	23517
RRID	AB_2187479
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P42285
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for SKIV2L2 antibody 12719-2-AP	Download protocol
IHC protocol for SKIV2L2 antibody 12719-2-AP	Download protocol
IF protocol for SKIV2L2 antibody 12719-2-AP	Download protocol
IP protocol for SKIV2L2 antibody 12719-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IP	Nat Commun TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV. Authors - Roy Matkovic View Article
human	IF	RNA Human Nuclear RNAi-Defective 2 (NRDE2) is an essential RNA splicing factor. Authors - Alan L Jiao View Article
human	WB,IP	Nature Nucleolar URB1 ensures 3' ETS rRNA removal to prevent exosome surveillance Authors - Lin Shan View Article
human	WB	Cell Genom NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors Authors - Yahui Wang View Article
		Nucleic Acids Res The NEXT complex regulates H3K27me3 levels to affect cancer progression by degrading G4/U-rich lncRNAs Authors - Qianqian Yang View Article
human	WB,IHC	Cell Death Differ hMTR4 promotes p53 protein degradation and tumor growth by accelerating rRNA processing and regulating the RPL5-MDM2 axis Authors - Chen Xie KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HepG2 using 12719-2-AP

WB analysis of human brain using 12719-2-AP

WB analysis of mouse testis using 12719-2-AP

WB analysis of HepG2 using 12719-2-AP

WB analysis of HepG2 using 12719-2-AP

IP experiment of HepG2 using 12719-2-AP

IHC staining of human stomach using 12719-2-AP

IF Staining of mouse testis using 12719-2-AP

SKIV2L2 Polyclonal antibody

SKIV2L2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.

Human Nuclear RNAi-Defective 2 (NRDE2) is an essential RNA splicing factor.

Nucleolar URB1 ensures 3' ETS rRNA removal to prevent exosome surveillance

NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors

The NEXT complex regulates H3K27me3 levels to affect cancer progression by degrading G4/U-rich lncRNAs

hMTR4 promotes p53 protein degradation and tumor growth by accelerating rRNA processing and regulating the RPL5-MDM2 axis