Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 19218-1-AP (same clone as 19218-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.
WB analysis of human skeletal muscle using 19218-1-AP (same clone as 19218-1-PBS)
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.
WB analysis of A431 using 19218-1-AP (same clone as 19218-1-PBS)
A431 cells were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.
WB analysis of A431 using 19218-1-AP (same clone as 19218-1-PBS)
A431 cells were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 19218-1-AP (same clone as 19218-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.
IP experiment of HepG2 using 19218-1-AP (same clone as 19218-1-PBS)
IP result of anti-SKIL (IP:19218-1-AP, 4ug; Detection:19218-1-AP 1:500) with HepG2 cells lysate 1600ug. This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.
IP experiment of HepG2 using 19218-1-AP (same clone as 19218-1-PBS)
IP result of anti-SKIL (IP:19218-1-AP, 4ug; Detection:19218-1-AP 1:500) with HepG2 cells lysate 1600ug. This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.
IHC staining of human endometrial cancer using 19218-1-AP (same clone as 19218-1-PBS)
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19218-1-AP (SKIL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.
IHC staining of human endometrial cancer using 19218-1-AP (same clone as 19218-1-PBS)
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19218-1-AP (SKIL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.
IHC staining of human endometrial cancer using 19218-1-AP (same clone as 19218-1-PBS)
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19218-1-AP (SKIL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 19218-1-AP (same clone as 19218-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SKIL antibody (19218-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 19218-1-AP (same clone as 19218-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SKIL antibody (19218-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

SKIL Polyclonal antibody, PBS Only

SKIL Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 19218-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19218-1-PBS targets SKIL in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5282 Product name: Recombinant human SKIL protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 335-684 aa of BC059386 Sequence: YLGTPEEKKLKIILEEMKEKFSMRSGKRNQSKTDAPSGMELQSWYPVIKQEGDHVSQTHSFLHPSYYLYMCDKVVAPNVSLTSAVSQSKELTKTEASKSISRQSEKAHSSGKLQKTVSYPDVSLEEQEKMDLKTSRELCSRLDASISNNSTSKRKSESATCNLVRDINKVGIGLVAAASSPLLVKDVICEDDKGKIMEEVMRTYLKQQEKLNLILQKKQQLQMEVKMLSSSKSMKELTEEQQNLQKELESLQNEHAQRMEEFYVEQKDLEKKLEQIMKQKCTCDSNLEKDKEAEYAGQLAELRQRLDHAEADRQELQDELRQEREARQKLEMMIKELKLQILKSSKTAKE 相同性解析による交差性が予測される生物種
Full Name	SKI-like oncogene
Calculated molecular weight	77 kDa
Observed molecular weight	77 kDa
GenBank accession number	BC059386
Gene Symbol	SKIL
Gene ID (NCBI)	6498
RRID	AB_10859824
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P12757
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

SKIL, also known as SnoN, is a mediator of the transforming growth factor-β (TGF-β) signaling pathway and exerts pro-oncogenic activity through inhibiting TGF-β/Smad pathway(PMID: 19114989). SKIL is involved in reduction of autophagy and subsequent tumor cell apoptosis in arsenic trioxide-treated promyelocytic leukemia and ovarian carcinoma. In addition, SKIL may regulate cell division or differentiation in response to extracellular signals.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 19218-1-AP (same clone as 19218-1-PBS)

WB analysis of human skeletal muscle using 19218-1-AP (same clone as 19218-1-PBS)

WB analysis of A431 using 19218-1-AP (same clone as 19218-1-PBS)

WB analysis of A431 using 19218-1-AP (same clone as 19218-1-PBS)

WB analysis of HepG2 using 19218-1-AP (same clone as 19218-1-PBS)

IP experiment of HepG2 using 19218-1-AP (same clone as 19218-1-PBS)

IP experiment of HepG2 using 19218-1-AP (same clone as 19218-1-PBS)

IHC staining of human endometrial cancer using 19218-1-AP (same clone as 19218-1-PBS)

IHC staining of human endometrial cancer using 19218-1-AP (same clone as 19218-1-PBS)

IHC staining of human endometrial cancer using 19218-1-AP (same clone as 19218-1-PBS)

IF Staining of HepG2 using 19218-1-AP (same clone as 19218-1-PBS)

IF Staining of HepG2 using 19218-1-AP (same clone as 19218-1-PBS)

SKIL Polyclonal antibody, PBS Only

SKIL Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information

Able™

Able^™