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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SKAR Polyclonal antibody (17466-1-AP)

    WB analysis of HeLa using 17466-1-AP (same clone as 17466-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using SKAR Polyclonal antibody (17466-1-AP)

    WB analysis of A549 using 17466-1-AP (same clone as 17466-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using SKAR Polyclonal antibody (17466-1-AP)

    WB analysis of HepG2 using 17466-1-AP (same clone as 17466-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using SKAR Polyclonal antibody (17466-1-AP)

    WB analysis of mouse brain using 17466-1-AP (same clone as 17466-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SKAR Polyclonal antibody (17466-1-AP)

    IP experiment of mouse brain using 17466-1-AP (same clone as 17466-1-PBS)

    IP result of anti-SKAR (IP:17466-1-AP, 4ug; Detection:17466-1-AP 1:300) with mouse brain tissue lysate 4000 ug. This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using SKAR Polyclonal antibody (17466-1-AP)

    IHC staining of human stomach using 17466-1-AP (same clone as 17466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17466-1-AP (SKAR antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using SKAR Polyclonal antibody (17466-1-AP)

    IHC staining of human stomach using 17466-1-AP (same clone as 17466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17466-1-AP (SKAR antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SKAR Polyclonal antibody (17466-1-AP)

    IF Staining of HeLa using 17466-1-AP (same clone as 17466-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17466-1-AP (SKAR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.

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SKAR Polyclonal antibody, PBS Only

SKAR Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 17466-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

POLDIP3, Polymerase delta-interacting protein 3, PDIP46, PDIP3, p46

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17466-1-PBS targets SKAR in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SKAR fusion protein Ag11338 相同性解析による交差性が予測される生物種
Full Name polymerase (DNA-directed), delta interacting protein 3
Calculated molecular weight 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC019643
Gene Symbol SKAR
Gene ID (NCBI) 84271
RRIDAB_2166620
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BY77
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.