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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse thymus tissue using SKAP55 Polyclonal antibody (14724-1-AP)

    WB analysis of mouse thymus using 14724-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14724-1-AP (SKAP55 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using SKAP55 Polyclonal antibody (14724-1-AP)

    WB analysis of Jurkat using 14724-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14724-1-AP (SKAP55 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SKAP55 Polyclonal antibody (14724-1-AP)

    IHC staining of human tonsillitis using 14724-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14724-1-AP (SKAP55 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SKAP55 Polyclonal antibody (14724-1-AP)

    IHC staining of human tonsillitis using 14724-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14724-1-AP (SKAP55 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SKAP55 Polyclonal antibody (14724-1-AP)

    IHC staining of human tonsillitis using 14724-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14724-1-AP (SKAP55 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SKAP55 Polyclonal antibody (14724-1-AP)

    IHC staining of human tonsillitis using 14724-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14724-1-AP (SKAP55 antibody at dilution of 1:50 (under 40x lens).

SKAP55 Polyclonal antibody

SKAP55 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14724-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

SKAP1, SCAP1, Src kinase-associated phosphoprotein of 55 kDa, Src kinase-associated phosphoprotein 1, Src family-associated phosphoprotein 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse thymus tissue, Jurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14724-1-AP targets SKAP55 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6467

Product name: Recombinant human SKAP55,SKAP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-358 aa of BC047870

Sequence: MQAAALPEEIRWLLEDAEEFLAEGLRNENLSAVARDHRDHILRGFQQIKARYYWDFQPQGGDIGQDSSDDNHSGTLGLSLTSDAPFLSDYQDEGMEDIVKGAQELDNVIKQGYLEKKSKDHSFFGSEWQKRWCVVSRGLFYYYANEKSKQPKGTFLIKGYGVRMAPHLRRDSKKESCFELTSQDRRSYEFTATSPAEARDWVDQISFLLKDLSSLTIPYEEDEEEEEKEETYDDIDGFDSPSCGSQCRPTILPGSVGIKEPTEEKEEEDIYEVLPDEEHDLEEDESGTRRKGDYASYYQGLWDCHGDQPDELSFQRGDLIRILSKEYNMYGWWVGELNSLVGIVPKEYLTTAFEVEER

相同性解析による交差性が予測される生物種
Full Name src kinase associated phosphoprotein 1
Calculated molecular weight 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC047870
Gene Symbol SKAP55
Gene ID (NCBI) 8631
RRIDAB_10858320
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86WV1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SKAP55 antibody 14724-1-APDownload protocol
IHC protocol for SKAP55 antibody 14724-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols