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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SKAP55 Polyclonal antibody

SKAP55 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14724-1-AP

Synonyms

pp55, SCAP1, SKAP 55, SKAP1, SKAP55

Validation Data Gallery

Applications:
  • WB analysis of mouse thymus using 14724-1-AP

    WB analysis of mouse thymus using 14724-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14724-1-AP (SKAP55 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 14724-1-AP

    WB analysis of Jurkat using 14724-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14724-1-AP (SKAP55 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 14724-1-AP

    IHC staining of human spleen using 14724-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14724-1-AP (SKAP55 antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human spleen using 14724-1-AP

    IHC staining of human spleen using 14724-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14724-1-AP (SKAP55 antibody at dilution of 1:50 (under 40x lens).

  • IHC staining of human tonsillitis using 14724-1-AP

    IHC staining of human tonsillitis using 14724-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14724-1-AP (SKAP55 antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human tonsillitis using 14724-1-AP

    IHC staining of human tonsillitis using 14724-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14724-1-AP (SKAP55 antibody at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse thymus tissue, Jurkat cells
Positive IHC detected inhuman spleen tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14724-1-AP targets SKAP55 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SKAP55 fusion protein Ag6467 相同性解析による交差性が予測される生物種
Full Name src kinase associated phosphoprotein 1
Calculated molecular weight 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC047870
Gene symbol SKAP1
Gene ID (NCBI) 8631
RRIDAB_10858320
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SKAP55 antibody 14724-1-APDownload protocol
IHC protocol for SKAP55 antibody 14724-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols