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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SIX4 Polyclonal antibody (21305-1-AP)

    WB analysis using 21305-1-AP (same clone as 21305-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21305-1-AP (SIX4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21305-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SIX4 Polyclonal antibody (21305-1-AP)

    WB analysis of HeLa using 21305-1-AP (same clone as 21305-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 21305-1-AP (SIX4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21305-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse tongue tissue using SIX4 Polyclonal antibody (21305-1-AP)

    IHC staining of mouse tongue using 21305-1-AP (same clone as 21305-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 21305-1-AP (SIX4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21305-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse salivary gland tissue using SIX4 Polyclonal antibody (21305-1-AP)

    IHC staining of mouse salivary gland using 21305-1-AP (same clone as 21305-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse salivary gland tissue slide using 21305-1-AP (SIX4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21305-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat salivary gland tissue using SIX4 Polyclonal antibody (21305-1-AP)

    IHC staining of rat salivary gland using 21305-1-AP (same clone as 21305-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat salivary gland tissue slide using 21305-1-AP (SIX4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21305-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using SIX4 Polyclonal antibody (21305-1-AP)

    IHC staining of human cervical cancer using 21305-1-AP (same clone as 21305-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21305-1-AP (SIX4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21305-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using SIX4 Polyclonal antibody (21305-1-AP)

    IHC staining of human ovary cancer using 21305-1-AP (same clone as 21305-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21305-1-AP (SIX4 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21305-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using SIX4 Polyclonal antibody (21305-1-AP)

    IHC staining of human ovary cancer using 21305-1-AP (same clone as 21305-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21305-1-AP (SIX4 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21305-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SIX4 Polyclonal antibody (21305-1-AP)

    IF Staining of HEK-293 using 21305-1-AP (same clone as 21305-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SIX4 antibody (21305-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 21305-1-PBS in a different storage buffer formulation.

SIX4 Polyclonal antibody, PBS Only

SIX4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21305-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

AREC3, Homeobox protein SIX4, SIX homeobox 4

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21305-1-PBS targets SIX4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15918

Product name: Recombinant human SIX4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 411-760 aa of BC098282

Sequence: TTTSYSPSVPVSFPGLIPSTEVKREGIQTVASQDGGSVVTFTTPVQINQYGIVQIPNSGANSQFLNGSIGFSPLQLPPVSVAASQGNISVSSSTSDGSTFTSESTTVQQGKVFLSSLAPSAVVYTVPNTGQTIGSVKQEGLERSLVFSQLMPVNQNAQVNANLSSENISGSGLHPLASSLVNVSPTHNFSLSPSTLLNPTELNRDIADSQPMSAPVASKSTVTSVSNTNYATLQNCSLITGQDLLSVPMTQAALGEIVPTAEDQVGHPSPAVHQDFVQEHRLVLQSVANMKENFLSNSESKATSSLMMLDSKSKYVLDGMVDTVCEDLETDKKELAKLQTVQLDEDMQDL

相同性解析による交差性が予測される生物種
Full Name SIX homeobox 4
Calculated molecular weight 781 aa, 83 kDa
Observed molecular weight 98 kDa
GenBank accession numberBC098282
Gene Symbol SIX4
Gene ID (NCBI) 51804
RRIDAB_10860258
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UIU6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The Six (sine oculis) proteins are a family of homeodomain transcription factors that share a conserved DNA-binding domain and are human homologs of the Drosophila sine oculis (so) protein. SIX homeobox 4 (SIX4), also known as AREC3, belongs to the the Six family. Six4 contains one Six domain (which funtions as a homeobox DNA-binding motif) and shares 90% sequence similarity with its mouse counterpart, suggesting that both proteins have similar DNA-binding properties. SIX4 is thought to be involved in neurogenesis, myogenesis, and development of other organs, based on its specific expression in certain neuronal cells of the developing embryo and in adult skeletal muscles.

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