Validation Data Gallery

Filter: AllWBIPIF/ICC

WB analysis of A2780 using 10709-1-AP
A2780 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of SKOV-3 using 10709-1-AP
SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of SKOV-3 using 10709-1-AP
SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10709-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10709-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10709-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 10709-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10709-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 10709-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10709-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 10709-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 10709-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 10709-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 10709-1-AP
IP result of anti-SIX1 (IP:10709-1-AP, 3ug; Detection:10709-1-AP 1:1000) with HEK-293 cells lysate 2560ug.
IF Staining of epithelial tumor cells using 10709-1-AP
IF result of anti-SIX1 (1:50) in p53-/- mammary epithelial tumor cells. Blue: hoechst nuclear stain, Red: SIX1 stain the mesenchymal cell which have undergone EMT.

Featured Product
KD/KO Validated

SIX1 Polyclonal antibody

SIX1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 10709-1-AP

Publications(33)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

TIP39, SIX homeobox 1, Sine oculis homeobox homolog 1, Homeobox protein SIX1, DFNA23

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (33)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A2780 cells, HEK-293 cells, HepG2 cells, L02 cells, PC-3 cells, SKOV-3 cells
Positive IP detected in	HEK-293 cells
Positive IF/ICC detected in	epithelial tumor cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 11 publications below
WB	See 23 publications below
IHC	See 13 publications below
IF	See 9 publications below
IP	See 2 publications below
CoIP	See 1 publications below
RIP	See 1 publications below

Product Information

10709-1-AP targets SIX1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1044 Product name: Recombinant human SIX1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-284 aa of BC008874 Sequence: MSMLPSFGFTQEQVACVCEVLQQGGNLERLGRFLWSLPACDHLHKNESVLKAKAVVAFHRGNFRELYKILESHQFSPHNHPKLQQLWLKAHYVEAEKLCGRPLGAVGKYRVRRKFPLPRTIWDGEETSYCFKEKSRGVLREWYAHNPYPSPREKRELAEATGLTTTQVSNWFKNRRQRDRAAEAKERENTENNNSSSNKQNQLSPLEGGKPLMSSSEEEFSPPQSPDQNSVLLLQGNMGHARSSNYSLPGLTASQPSHGLQTHQHQLQDSLLGPLTSSLVDLGS 相同性解析による交差性が予測される生物種
Full Name	SIX homeobox 1
Calculated molecular weight	32 kDa
Observed molecular weight	32-35 kDa
GenBank accession number	BC008874
Gene Symbol	SIX1
Gene ID (NCBI)	6495
RRID	AB_2189077
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q15475
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SIX1 belongs to SIX family that contains a divergent DNA-binding homeodomain and an upstream SIX domain, which may participate both in determining DNA-binding specificity and in mediating protein-protein interactions. It may be involved in limb tendon and ligament development, and specifically expressed in skeletal muscle. SIX1 plays a essential role in the development of numerous organs and shows littlt to no expression in most non-neoplastic adult tissue, yet is overexpression in a number of meoplasms where it increases cell proliferation and survival. The calcualted molecular weight of SIX1 is 32kDa, but modified SIX1 is about 35 kDa.

Protocols

Product Specific Protocols
IP protocol for SIX1 antibody 10709-1-AP	Download protocol
WB protocol for SIX1 antibody 10709-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Cancer Cell Transcriptional Regulation of the Warburg Effect in Cancer by SIX1. Authors - Ling Li KO Validated View Article
human,mouse	WB,IHC	Adv Sci (Weinh) Transcriptional Regulation of De Novo Lipogenesis by SIX1 in Liver Cancer Cells Authors - Ling Li View Article
mouse	WB	Nat Commun Muscle progenitor specification and myogenic differentiation are associated with changes in chromatin topology. Authors - Nan Zhang View Article
mouse		PLoS Biol Dynamic enhancers control skeletal muscle identity and reprogramming. Authors - Krithika Ramachandran View Article
human	WB,IHC,RIP	Int J Biol Sci Novel LncRNA LINC02936 Suppresses Ferroptosis and Promotes Tumor Progression by Interacting with SIX1/CP Axis in Endometrial Cancer Authors - Zihui Zhang KD Validated View Article
human	WB	Theranostics O-GlcNAcylation of SIX1 enhances its stability and promotes Hepatocellular Carcinoma Proliferation. Authors - Yi Chu KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A2780 using 10709-1-AP

WB analysis of SKOV-3 using 10709-1-AP

WB analysis of SKOV-3 using 10709-1-AP

WB analysis of HEK-293 using 10709-1-AP

WB analysis of HEK-293 using 10709-1-AP

WB analysis of HepG2 using 10709-1-AP

WB analysis of L02 using 10709-1-AP

WB analysis of HEK-293 using 10709-1-AP

WB analysis of PC-3 using 10709-1-AP

WB analysis of HepG2 using 10709-1-AP

WB analysis of PC-3 using 10709-1-AP

WB analysis of L02 using 10709-1-AP

WB analysis of L02 using 10709-1-AP

IP experiment of HEK-293 using 10709-1-AP

IF Staining of epithelial tumor cells using 10709-1-AP

SIX1 Polyclonal antibody

SIX1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.

Transcriptional Regulation of De Novo Lipogenesis by SIX1 in Liver Cancer Cells

Muscle progenitor specification and myogenic differentiation are associated with changes in chromatin topology.

Dynamic enhancers control skeletal muscle identity and reprogramming.

Novel LncRNA LINC02936 Suppresses Ferroptosis and Promotes Tumor Progression by Interacting with SIX1/CP Axis in Endometrial Cancer

O-GlcNAcylation of SIX1 enhances its stability and promotes Hepatocellular Carcinoma Proliferation.