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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SIRT7 Recombinant monoclonal antibody (84805-4-RR)

    WB analysis using 84805-4-RR (same clone as 84805-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84805-4-RR (SIRT7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84805-4-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293T cells using SIRT7 Recombinant monoclonal antibody (84805-4-RR)

    IP experiment of HEK-293T using 84805-4-RR (same clone as 84805-4-PBS)

    IP result of anti-SIRT7 (IP:84805-4-RR, 4ug; Detection:84805-4-RR 1:2000) with HEK-293T cells lysate 2200 ug. This data was developed using the same antibody clone with 84805-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using SIRT7 Recombinant monoclonal antibody (84805-4-RR)

    IHC staining of rat liver using 84805-4-RR (same clone as 84805-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84805-4-RR (SIRT7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84805-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using SIRT7 Recombinant monoclonal antibody (84805-4-RR)

    IHC staining of rat liver using 84805-4-RR (same clone as 84805-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84805-4-RR (SIRT7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84805-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SIRT7 Recombinant monoclonal antibody (84805-4-RR)

    IF Staining of MCF-7 using 84805-4-RR (same clone as 84805-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SIRT7 antibody (84805-4-RR, Clone: 242167C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84805-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84805-4-RR

    Affinity and Kinetic Characterization of 84805-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84805-4-RR against Human SIRT7 were performed. The affinity constant is below 1 pM.

SIRT7 Recombinant monoclonal antibody, PBS Only

SIRT7 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 84805-4-PBS
Clone No.242167C9

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

242167C9, EC:2.3.1.-, EC:2.3.1.286, NAD-dependent protein deacylase sirtuin-7, SIR2 like protein 7

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84805-4-PBS targets SIRT7 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag31794

Product name: Recombinant human SIRT7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-400 aa of NM_016538

Sequence: MAAGGLSRSERKAAERVRRLREEQQRERLRQVSRILRKAAAERSAEEGRLLAESADLVTELQGRSRRREGLKRRQEEVCDDPEELRGKVRELASAVRNAKYLVVYTGAGISTAASIPDYRGPNGVWTLLQKGRSVSAADLSEAEPTLTHMSITRLHEQKLVQHVVSQNCDGLHLRSGLPRTAISELHGNMYIEVCTSCVPNREYVRVFDVTERTALHRHQTGRTCHKCGTQLRDTIVHFGERGTLGQPLNWEAATEAASRADTILCLGSSLKVLKKYPRLWCMTKPPSRRPKLYIVNLQWTPKDDWAALKLHGKCDDVMRLLMAELGLEIPAYSRWQDPIFSLATPLRAGEEGSHSRKSLCRSREEAPPGDRGAPLSSAPILGGWFGRGCTKRTKRKKVT

相同性解析による交差性が予測される生物種
Full Name sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae)
Calculated molecular weight45KD
Observed molecular weight 45 kDa
GenBank accession numberNM_016538
Gene Symbol SIRT7
Gene ID (NCBI) 51547
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9NRC8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SIRT7 (NAD-dependent deacetylase sirtuin-7), also known as SIR2L7, is a member of the class IV sirtuin family and is localized to the nucleolus. Expressed throughout the body, SIRT7 associates with rDNA genes where it interacts with histones and acts as a positive regulator of RNA polymerase I (Pol I). SIRT7 is a probable NAD-dependent deacetylase whose expression is upregulated in thyroid carcinoma cells. Overexpression of SIRT7 increases Pol I-mediated transcription, thereby speeding cell growth and contributing to the development of cancer.

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