Validation Data Gallery
Tested Applications
| Positive WB detected in | HCT 116 cells, BxPC-3 cells, HepG2 cells, RAW 264.7 cells, HSC-T6 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87677-3-RR targets SIRT6 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag4469 Product name: Recombinant human SIRT6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-328 aa of BC028220 Sequence: MSVNYAAGLSPYADKGKCGLPEIFDPPEELERKVWELARLVWQSSNVVFHTGAGISTASGIPDFRGPHGVWTMEERGLAPKFDTTFESARPTQTHMALVQLERVGLLRFLVSQNVDGLHVRSGFPRDKLAELHGNMFVEECAKCKTQYVRDTVVGTMGLKATGRLCTVAKARGLRACRNADLSITLGTSLQIRPSGNLPLATKRRGGRLVIVNLQPTKHDRHADLRIHGYVDEVMTRLMKHLGLEIPAWDGPRVLERALPPLPRPPTPKLEPKEESPTRINGSIPAGPKQEPCAQHNGSEPASPKRERPTSPAPHRPPKRVKAKAVPS 相同性解析による交差性が予測される生物種 |
| Full Name | sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) |
| Calculated molecular weight | 39 kDa |
| Observed molecular weight | 37-45 kDa |
| GenBank accession number | BC028220 |
| Gene Symbol | SIRT6 |
| Gene ID (NCBI) | 51548 |
| RRID | AB_3745691 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8N6T7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SIRT6, also named as SIR2L6, belongs to the sirtuin family. It is known to deacetylate H3K9, H3K56 and CtIP in vivo. SIRT6 regulates the expression of a large number of stress-responsive and metabolism related genes, promotes genomic stability and stimulates base excision and double strand break DNA repair(PMID: 20729089). By maintaining both the integrity and the expression of the mammalian genome, SIRT6 thus serves several roles that parallel Sir2 function(PMID: 20729089). In addtion, Sirt6-deficient animals develop normally for several weeks after birth, but succumb to an acute multi-organ degenerative syndrome that is uniformly lethal by one month of age(PMID: 19135889).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SIRT6 antibody 87677-3-RR | Download protocol |
| IP protocol for SIRT6 antibody 87677-3-RR | Download protocol |
| WB protocol for SIRT6 antibody 87677-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |