Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue, mouse liver tissue |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28148-1-AP targets SIRT4 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28003 Product name: Recombinant human SIRT4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 149-306 aa of BC109319 Sequence: HTKAGSRRLTELHGCMDRVLCLDCGEQTPRGVLQERFQVLNPTWSAEAHGLAPDGDVFLSEEQVRSFQVPTCVQCGGHLKPDVVFFGDTVNPDKVDFVHKRVKEADSLLVVGSSLQVYSGYRFILTAWEKKLPIAILNIGPTRSDDLACLKLNSRCGE 相同性解析による交差性が予測される生物種 |
| Full Name | sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae) |
| Calculated molecular weight | 314 aa, 35 kDa |
| Observed molecular weight | 30-35 kDa |
| GenBank accession number | BC109319 |
| Gene Symbol | SIRT4 |
| Gene ID (NCBI) | 23409 |
| RRID | AB_3742279 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6E7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SIRT4, also named as NAD-dependent protein lipoamidase sirtuin-4, mitochondria, is a 314 amino acid protein, which belongs to the sirtuin family. Class II subfamily. SIRT4 is detected in vascular smooth muscle and striated muscle. SIRT4 is detected in insulin-producing beta-cells in pancreas islets of Langerhans. SIRT4 Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. It catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications and inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner. SIRT4 expression is down-regulated in a number of cancers, while overexpression reduces cell proliferation, transformation, and tumor development.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SIRT4 antibody 28148-1-AP | Download protocol |
| WB protocol for SIRT4 antibody 28148-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |