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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SIRT3 Polyclonal antibody

SIRT3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29838-1-AP

Synonyms

hSIRT3, SIR2 like protein 3, SIR2L3, SIRT3

Validation Data Gallery

Applications:
  • WB analysis using 29838-1-AP

    WB analysis using 29838-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29838-1-AP (SIRT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 29838-1-AP

    IHC staining of human prostate cancer using 29838-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29838-1-AP (SIRT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 29838-1-AP

    IHC staining of human prostate cancer using 29838-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29838-1-AP (SIRT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

29838-1-AP targets SIRT3 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIRT3 fusion protein Ag31333 相同性解析による交差性が予測される生物種
Full Name sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
Calculated molecular weight 44 kDa
Observed molecular weight28-30 kDa
GenBank accession numberBC001042
Gene symbol SIRT3
Gene ID (NCBI) 23410
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SIRT3 antibody 29838-1-APDownload protocol
IHC protocol for SIRT3 antibody 29838-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols