Validation Data Gallery
Tested Applications
| Positive WB detected in | U-937 cells, A375 cells, PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 2 publications below |
Product Information
11811-1-AP targets SIRP beta 1/CD172b in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2478 Product name: Recombinant human SIRPB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-181 aa of BC025286 Sequence: MPVPASWPHLPSPFLLMTLLLGRLTGVAGEDELQVIQPEKSVSVAAGESATLRCAMTSLIPVGPIMWFRGAGAGRELIYNQKEGHFPRVTTVSELTKRNNLDFSISISNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVREPALAPTAPLLVALLLGPKLLLVVGVSAIYICWKQKA 相同性解析による交差性が予測される生物種 |
| Full Name | signal-regulatory protein beta 1 |
| Calculated molecular weight | 43 kDa |
| Observed molecular weight | 43 kDa |
| GenBank accession number | BC025286 |
| Gene Symbol | SIRPB1 |
| Gene ID (NCBI) | 10326 |
| RRID | AB_2188197 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00241 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SIRP Beta 1 is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are cell surface signaling receptors differentially expressed in leukocytes and the central nervous system. SIRP Beta 1 interacts with TYROBP/DAP12, a protein bearing immunoreceptor tyrosine-based activation motifs. SIRP Beta 1 also participates in the recruitment of tyrosine kinase SYK.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SIRP beta 1/CD172b antibody 11811-1-AP | Download protocol |
| WB protocol for SIRP beta 1/CD172b antibody 11811-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
PLoS One SNPs array karyotyping reveals a novel recurrent 20p13 amplification in primary myelofibrosis. | ||
Neuropathology Immunohistochemical characterization of microglia in Nasu-Hakola disease brains. |
