• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using SIRP Alpha/CD172a Recombinant antibody (84430-2-RR)

    WB analysis using 84430-2-RR (same clone as 84430-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84430-2-RR (SIRP alpha/CD172a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84430-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SIRP Alpha/CD172a Recombinant antibody (84430-2-RR)

    WB analysis using 84430-2-RR (same clone as 84430-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84430-2-RR (SIRP alpha/CD172a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84430-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01311-4

    Sandwich ELISA standard curve of MP01311-4

    Sandwich ELISA standard curve of MP01311-4, Mouse SIRP alpha/CD172a Recombinant Matched Antibody Pair - PBS only. 84430-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1520. 84430-2-PBS was HRP conjugated as the detection antibody. Range: 19.5-625 pg/mL.

  • Sample test of MP01311-4

    Sample test of MP01311-4

    The mean SIRP alpha/CD172a concentration was determined to be 1,352.0 ng/mL in mouse brain tissue extract based on a 2.0 mg/mL extract load, 1,425.8 ng/mL in mouse cerebellum tissue extract based on a 2.9 mg/mL extract load, 796.6 ng/mL in RAW 264.7 cell extract based on a 4.2 mg/mL extract load and 4.0 ng/mL in NIH/3T3 cell extract based on a 4.4 mg/mL extract load.

  • Sample test of MP01311-4

    Sample test of MP01311-4

    The mean SIRP alpha/CD172a concentration was determined to be 595.2 ng/mL in mouse brain tissue homogenate based on a 2.2 mg/mL extract load.

  • Sample test of MP01311-4

    Sample test of MP01311-4

    Serum of six mice was measured. The SIRP alpha/CD172a concentration of detected samples was determined to be 69.0 ng/mL with a range of 59.9-73.9 ng/mL.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SIRP Alpha/CD172a Recombinant antibody (84430-2-RR)

    IHC staining of mouse brain using 84430-2-RR (same clone as 84430-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84430-2-RR (SIRP Alpha/CD172a antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84430-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SIRP Alpha/CD172a Recombinant antibody (84430-2-RR)

    IHC staining of mouse brain using 84430-2-RR (same clone as 84430-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84430-2-RR (SIRP Alpha/CD172a antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84430-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using SIRP Alpha/CD172a Recombinant antibody (84430-2-RR)

    IF Staining of RAW 264.7 using 84430-2-RR (same clone as 84430-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using SIRP alpha/CD172a antibody (84430-2-RR, Clone: 241794D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84430-2-PBS in a different storage buffer formulation.

Mouse SIRP Alpha/CD172a Recombinant monoclonal antibody, PBS Only (Detector)

SIRP Alpha/CD172a Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84430-2-PBS
Clone No.241794D9

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

241794D9, Bit, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, CD172a

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84430-2-PBS targets SIRP Alpha/CD172a as part of a matched antibody pair:

MP01311-4: 84430-3-PBS capture and 84430-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg1520

Product name: Recombinant Mouse SIRP alpha/CD172a protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 32-373 aa of NM_007547.4

Sequence: KELKVTQPEKSVSVAAGDSTVLNCTLTSLLPVGPIRWYRGVGPSRLLIYSFAGEYVPRIRNVSDTTKRNNMDFSIRISNVTPADAGIYYCVKFQKGSSEPDTEIQSGGGTEVYVLAKPSPPEVSGPADRGIPDQKVNFTCKSHGFSPRNITLKWFKDGQELHPLETTVNPSGKNVSYNISSTVRVVLNSMDVNSKVICEVAHITLDRSPLRGIANLSNFIRVSPTVKVTQQSPTSMNQVNLTCRAERFYPEDLQLIWLENGNVSRNDTPKNLTKNTDGTYNYTSLFLVNSSAHREDVVFTCQVKHDQQPAITRNHTVLGFAHSSDQGSMQTFPDNNATHNWN

相同性解析による交差性が予測される生物種
Full Name signal-regulatory protein alpha
Calculated molecular weight56 kDa
Observed molecular weight90-140 kDa
GenBank accession numberNM_007547.4
Gene Symbol Sirpa
Gene ID (NCBI) 19261
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP97797-2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).

||
New chat Able
正在加载，请稍候...