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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SIM2 Polyclonal antibody (21069-1-AP)

    WB analysis using 21069-1-AP (same clone as 21069-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21069-1-AP (SIM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21069-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using SIM2 Polyclonal antibody (21069-1-AP)

    WB analysis of human skeletal muscle using 21069-1-AP (same clone as 21069-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21069-1-AP (SIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21069-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse skeletal muscle tissue using SIM2 Polyclonal antibody (21069-1-AP)

    IP experiment of mouse skeletal muscle using 21069-1-AP (same clone as 21069-1-PBS)

    IP result of anti-SIM2 (IP:21069-1-AP, 4ug; Detection:21069-1-AP 1:800) with mouse skeletal muscle tissue lysate 2200ug. This data was developed using the same antibody clone with 21069-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SIM2 Polyclonal antibody (21069-1-AP)

    IHC staining of mouse testis using 21069-1-AP (same clone as 21069-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21069-1-AP (SIM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21069-1-PBS in a different storage buffer formulation.

SIM2 Polyclonal antibody, PBS Only

SIM2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 21069-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Class E basic helix-loop-helix protein 15, SIM, Single minded homolog 2, Single-minded homolog 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21069-1-PBS targets SIM2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15204

Product name: Recombinant human SIM2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 342-525 aa of BC110444

Sequence: LSLEQVSTAKSQDSWRTALSTSQETRKLVKPKNTKMKTKLRTNPYPPQQYSSFQMDKLECGQLGNWRASPPASAAAPPELQPHSESSDLLYTPSYSLPFSYHYGHFPLDSHVFSSKKPMLPAKFGQPQGSPCEVARFFLSTLPASGECQWHYANPLVPSSSSPAKNPPEPPANTARHSLVPSYE

相同性解析による交差性が予測される生物種
Full Name single-minded homolog 2 (Drosophila)
Calculated molecular weight 667 aa, 73 kDa
Observed molecular weight 60-64 kDa
GenBank accession numberBC110444
Gene Symbol SIM2
Gene ID (NCBI) 6493
RRIDAB_10732844
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14190
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SIM2, also named as single-minded homolog 2, is a 667 amino acid protein, which contains 1 bHLH (basic helix-loop-helix) domain and one PAS-associated C-terminal domain. SIM2 localizes in the nucleus and has tow isoforms. Isoform SIM2 is a 73 kDa protein and Isoform SIM2S is 63 kDa protein. SIM2 as a transcription factor may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development.

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