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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SIKE1 Polyclonal antibody

SIKE1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 22432-1-AP

Synonyms

RP5 1000E10.4, RP5-1000E10.4, SIKE, SIKE1, Suppressor of IKBKE 1, suppressor of IKK epsilon

Validation Data Gallery

Applications:
  • WB analysis of SH-SY5Y using 22432-1-AP

    WB analysis of SH-SY5Y using 22432-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22432-1-AP (SIKE1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 22432-1-AP

    WB analysis of MCF-7 using 22432-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 22432-1-AP (SIKE1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 22432-1-AP

    WB analysis of mouse brain using 22432-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22432-1-AP (SIKE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 22432-1-AP

    WB analysis of HEK-293 using 22432-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22432-1-AP (SIKE1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inSH-SY5Y cells, HEK-293 cells, MCF-7 cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

22432-1-AP targets SIKE1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIKE1 fusion protein Ag18196 相同性解析による交差性が予測される生物種
Full Name suppressor of IKK epsilon
Calculated molecular weight 207 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC005934
Gene symbol RP5-1000E10.4
Gene ID (NCBI) 80143
RRIDAB_10914502
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SIKE1 antibody 22432-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols