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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SIK2 Polyclonal antibody (27608-1-AP)

    WB analysis using 27608-1-AP (same clone as 27608-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27608-1-AP (SIK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27608-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using SIK2 Polyclonal antibody (27608-1-AP)

    IHC staining of human ovary cancer using 27608-1-AP (same clone as 27608-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27608-1-AP (SIK2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27608-1-PBS in a different storage buffer formulation.

SIK2 Polyclonal antibody, PBS Only

SIK2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27608-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

SIK 2, salt inducible kinase 2, Qin induced kinase, QIK, LOH11CR1I

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27608-1-PBS targets SIK2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26468

Product name: Recombinant human SIK2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 617-926 aa of BC113459

Sequence: VQLLYEQIGPEADPNLAPAAPQLQDLASSCPQEEVSQQQESVSTLPASVHPQLSPRQSLETQYLQHRLQKPSLLSKAQNTCQLYCKEPPRSLEQQLQEHRLQQKRLFLQKQSQLQAYFNQMQIAESSYPQPSQQLPLPRQETPPPSQQAPPFSLTQPLSPVLEPSSEQMQYSPFLSQYQEMQLQPLPSTSGPRAAPPLPTQLQQQQPPPPPPPPPPRQPGAAPAPLQFSYQTCELPSAASPAPDYPTPCQYPVDGAQQSDLTGPDCPRSPGLQEAPSSYDPLALSELPGLFDCEMLDAVDPQHNGYVLVN

相同性解析による交差性が予測される生物種
Full Name salt-inducible kinase 2
Calculated molecular weight 926 aa, 104 kDa
Observed molecular weight103-110 kDa
GenBank accession numberBC113459
Gene Symbol SIK2
Gene ID (NCBI) 23235
RRIDAB_3669614
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0K1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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