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  • Featured Product
  • KD/KO Validated

SIK1 Polyclonal antibody

SIK1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17370-1-AP

Synonyms

MSK, salt inducible kinase 1, SIK, SIK 1, SIK1, SNF1LK

Validation Data Gallery

Applications:
  • IHC staining of human testis using 17370-1-AP

    IHC staining of human testis using 17370-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17370-1-AP (SIK1 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human testis using 17370-1-AP

    IHC staining of human testis using 17370-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17370-1-AP (SIK1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon cancer using 17370-1-AP

    IHC staining of human colon cancer using 17370-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17370-1-AP (SIK1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon cancer using 17370-1-AP

    IHC staining of human colon cancer using 17370-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17370-1-AP (SIK1 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human stomach using 17370-1-AP

    IHC staining of human stomach using 17370-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17370-1-AP (SIK1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human stomach using 17370-1-AP

    IHC staining of human stomach using 17370-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17370-1-AP (SIK1 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive IHC detected inhuman testis tissue, human stomach tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 17370-1-AP is SIK1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIK1 fusion protein Ag10954 相同性解析による交差性が予測される生物種
Full Name salt-inducible kinase 1
Calculated molecular weight 783 aa, 85 kDa
GenBank accession numberBC038504
Gene symbol SIK1
Gene ID (NCBI) 150094
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SIK1(Salt-inducible kinase 1) is also named as SIK, SNF1LK and belongs to the CAMK Ser/Thr protein kinase family. It associates constitutively with the NK regulatory complex and is responsible for increases in its catalytic activity following small elevations in intracellular sodium concentrations(PMID:17939993). SIK1 is required for phosphorylation of class II HDACs and for expression of MEF2 target genes in myocytes(PMID:17468767). Defects in SIK1 may be associated with some cancers, such as breast cancers. Loss of SIK1 correlates with poor patient outcome in breast cancers(PMID:19622832).

Protocols

Product Specific Protocols
IHC protocol for SIK1 antibody 17370-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

PLoS One

Salt-inducible kinases (SIK) inhibition reduces RANKL-induced osteoclastogenesis.

Authors - Maria Stella Lombardi
  • KO Validated
View Article