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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse spleen tissue using SIGNR1/CD209b Recombinant antibody (84365-5-RR)

    WB analysis of mouse spleen using 84365-5-RR

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 84365-5-RR (SIGNR1/CD209b antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using SIGNR1/CD209b Recombinant antibody (84365-5-RR)

    IHC staining of mouse spleen using 84365-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84365-5-RR (SIGNR1/CD209b antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using SIGNR1/CD209b Recombinant antibody (84365-5-RR)

    IHC staining of mouse spleen using 84365-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84365-5-RR (SIGNR1/CD209b antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using SIGNR1/CD209b Recombinant antibody (84365-5-RR)

    IF Staining of mouse spleen using 84365-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using SIGNR1/CD209b antibody (84365-5-RR, Clone: 241726B1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84365-5-RR

    Affinity and Kinetic Characterization of 84365-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84365-5-RR against Mouse SIGNR1/CD209b were performed. The affinity constant is 0.155 nM.

SIGNR1/CD209b Recombinant monoclonal antibody

SIGNR1/CD209b Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 84365-5-RR
Clone No.241726B1

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

241726B1, CD209, CD209 antigen-like protein B, Cd209b, DC-SIGNR1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse spleen tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84365-5-RR targets SIGNR1/CD209b in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg1497

Product name: Recombinant Mouse SIGNR1/CD209b protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: N-6*His

Domain: 74-325 aa of NM_026972.5

Sequence: QVSKTPNTERQKEQEKILQELTQLTDELTSRIPISQGKNESMQAKITEQLMQLKTELLSRIPIFQGQNESIQEKISEQLMQLKAELLSKISSFPVKDDSKQEKIYQQLVQMKTELFRLCRLCPWDWTFLLGNCYFFSKSQRNWNDAVTACKEVKAQLVIINSDEEQTFLQQTSKAKGPTWMGLSDLKKEATWLWVDGSTLSSRFQKYWNRGEPNNIGEEDCVEFAGDGWNDSKCELKKFWICKKSATPCTEG

相同性解析による交差性が予測される生物種
Full Name CD209b antigen
Calculated molecular weight37 kDa
Observed molecular weight45-50 kDa
GenBank accession numberNM_026972.5
Gene Symbol Cd209b
Gene ID (NCBI) 69165
RRIDAB_3671901
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ8CJ91-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SIGNR1 (CD209b) is a mouse homolog of the human DC-SIGN receptor (PMID: 11581173). SIGNR1 is a type II transmembrane protein with an extracellular domain consisting of a neck region made up of four repeats of 28 amino acids and a carbohydrate recognition domain (PMID: 15583012). It is expressed on marginal zone macrophages and peritoneal macrophages and is a key receptor for the recognition and efficient clearance of pathogens by the innate immune system.

Protocols

Product Specific Protocols
IF protocol for SIGNR1/CD209b antibody 84365-5-RRDownload protocol
IHC protocol for SIGNR1/CD209b antibody 84365-5-RRDownload protocol
WB protocol for SIGNR1/CD209b antibody 84365-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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