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Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using Siglec-9 Polyclonal antibody (13377-1-AP)

    WB analysis of HL-60 using 13377-1-AP (same clone as 13377-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 13377-1-AP (Siglec-9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13377-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using Siglec-9 Polyclonal antibody (13377-1-AP)

    WB analysis of mouse skeletal muscle using 13377-1-AP (same clone as 13377-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13377-1-AP (Siglec-9 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13377-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using Siglec-9 Polyclonal antibody (13377-1-AP)

    WB analysis of U-937 using 13377-1-AP (same clone as 13377-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 13377-1-AP (Siglec-9 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13377-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A375 cells using Siglec-9 Polyclonal antibody (13377-1-AP)

    WB analysis of A375 using 13377-1-AP (same clone as 13377-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 13377-1-AP (Siglec-9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13377-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Siglec-9 Polyclonal antibody (13377-1-AP)

    IHC staining of human liver cancer using 13377-1-AP (same clone as 13377-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13377-1-AP (Siglec-9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13377-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Siglec-9 Polyclonal antibody (13377-1-AP)

    IHC staining of human liver cancer using 13377-1-AP (same clone as 13377-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13377-1-AP (Siglec-9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13377-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using Siglec-9 Polyclonal antibody (13377-1-AP)

    IF Staining of human tonsillitis using 13377-1-AP (same clone as 13377-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using Siglec-9 antibody (13377-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13377-1-PBS in a different storage buffer formulation.

Siglec-9 Polyclonal antibody, PBS Only

Siglec-9 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 13377-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

SIGLEC9, CD329, Protein FOAP 9, Sialic acid-binding Ig-like lectin 9, Siglec 9

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13377-1-PBS targets Siglec-9 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4201

Product name: Recombinant human SIGLEC9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 211-341 aa of BC035365

Sequence: LTCQVTFPGASVTTNKTVHLNVSYPPQNLTMTVFQGDGTVSTVLGNGSSLSLPEGQSLRLVCAVDAVDSNPPARLSLSWRGLTLCPSQPSNPGVLELPWVHLRDEAEFTCRAQNPLGSQQVYLNVSLQSKA

相同性解析による交差性が予測される生物種
Full Name sialic acid binding Ig-like lectin 9
Calculated molecular weight 463 aa, 50 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC035365
Gene Symbol Siglec-9
Gene ID (NCBI) 27180
RRIDAB_2187293
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y336
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Sialic acid binding Ig-like lectin 9 (Siglec-9), also known as CD329, is a member of the Siglec family of glycan-recognition proteins. Siglec-9 is a type-I transmembrane protein consisting of an N-terminal extracellular region that contains an N-terminal V-set domain and two C2-set domains, a transmembrane region, and an intracellular domain with an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an ITIM-like motif (PMID: 10801862). It is expressed quite broadly among human blood leukocytes, including monocytes, neutrophils, B cells, NK cells, and minor subsets of T cells (PMID: 32322597). Siglec-9 functions as an inhibitory immune checkpoint and can be targeted to enhance therapeutic antitumor immunity (PMID: 34155121; 37460871).

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