Validation Data Gallery
Tested Applications
| Positive WB detected in | HL-60 cells, THP-1 cells, U-937 cells |
| Positive IF/ICC detected in | U-937 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86628-3-RR targets SIGLEC14 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg4178 Product name: Recombinant Human SIGLEC14 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 17-358 aa of NM_001098612.1 Sequence: EKPVYELQVQKSVTVQEGLCVLVPCSFSYPWRSWYSSPPLYVYWFRDGEIPYYAEVVATNNPDRRVKPETQGRFRLLGDVQKKNCSLSIGDARMEDTGSYFFRVERGRDVKYSYQQNKLNLEVTALIEKPDIHFLEPLESGRPTRLSCSLPGSCEAGPPLTFSWTGNALSPLDPETTRSSELTLTPRPEDHGTNLTCQVKRQGAQVTTERTVQLNVSYAPQNLAISIFFRNGTGTALRILSNGMSVPIQEGQSLFLACTVDSNPPASLSWFREGKALNPSQTSMSGTLELPNIGAREGGEFTCRVQHPLGSQHLSFILSVQRSSSSCICVTEKQQGSWPLVL 相同性解析による交差性が予測される生物種 |
| Full Name | sialic acid binding Ig-like lectin 14 |
| Calculated molecular weight | 44 kDa |
| Observed molecular weight | 44 kDa |
| GenBank accession number | NM_001098612.1 |
| Gene Symbol | SIGLEC14 |
| Gene ID (NCBI) | 100049587 |
| RRID | AB_3745019 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q08ET2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Siglecs are involved in regulating cell proliferation, differentiation, apoptosis, and implementing cell-cell interactions (PMID: 19076345). They play important roles in regulating immune responses, in hostpathogen interactions, and in tumorigenesis (PMID: 22524423; 25234143). Sialic acid-binding Ig-like lectin 14 (SIGLEC14) is expressed by granulocytes and monocytes, which has an activating role in regulating macrophage immune responses (PMID: 24799499; 37024598).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SIGLEC14 antibody 86628-3-RR | Download protocol |
| WB protocol for SIGLEC14 antibody 86628-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |