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Validation Data Gallery

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  • Western Blot (WB) analysis of TF-1 cells using SIGLEC10 Monoclonal antibody (60547-1-Ig)

    WB analysis of TF-1 using 60547-1-Ig

    TF-1 cells were subjected to SDS PAGE followed by western blot with 60547-1-Ig (SIGLEC10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Karpas-422 cells using SIGLEC10 Monoclonal antibody (60547-1-Ig)

    WB analysis of Karpas-422 using 60547-1-Ig

    Karpas-422 cells were subjected to SDS PAGE followed by western blot with 60547-1-Ig (SIGLEC10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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SIGLEC10 Monoclonal antibody

SIGLEC10 Monoclonal Antibody for WB, ELISA
Cat No. 60547-1-Ig
Clone No.2E9D7

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, ELISA

Synonyms

Siglec-10, Siglec-like protein 2, Siglec like protein 2, SIGLEC 10, Sialic acid-binding Ig-like lectin 10

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inKarpas-422 cells, TF-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60547-1-Ig targets SIGLEC10 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SIGLEC10 fusion protein Ag20755 相同性解析による交差性が予測される生物種
Full Name sialic acid binding Ig-like lectin 10
Calculated molecular weight 697 aa, 77 kDa
Observed molecular weight70-100 kDa
GenBank accession numberBC101725
Gene Symbol SIGLEC10
Gene ID (NCBI) 89790
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96LC7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SIGLEC10 antibody 60547-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols