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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SIAH1 Polyclonal antibody (13886-1-AP)

    WB analysis of HeLa using 13886-1-AP (same clone as 13886-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using SIAH1 Polyclonal antibody (13886-1-AP)

    WB analysis of A549 using 13886-1-AP (same clone as 13886-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using SIAH1 Polyclonal antibody (13886-1-AP)

    WB analysis of Jurkat using 13886-1-AP (same clone as 13886-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW264.7 using SIAH1 Polyclonal antibody (13886-1-AP)

    WB analysis of RAW264.7 using 13886-1-AP (same clone as 13886-1-PBS)

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse small intestine tissue using SIAH1 Polyclonal antibody (13886-1-AP)

    WB analysis of mouse small intestine using 13886-1-AP (same clone as 13886-1-PBS)

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SIAH1 Polyclonal antibody (13886-1-AP)

    WB analysis of HeLa using 13886-1-AP (same clone as 13886-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using SIAH1 Polyclonal antibody (13886-1-AP)

    WB analysis of COLO 320 using 13886-1-AP (same clone as 13886-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using SIAH1 Polyclonal antibody (13886-1-AP)

    WB analysis of HepG2 using 13886-1-AP (same clone as 13886-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SIAH1 Polyclonal antibody (13886-1-AP)

    IHC staining of mouse testis using 13886-1-AP (same clone as 13886-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13886-1-AP (SIAH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SIAH1 Polyclonal antibody (13886-1-AP)

    IHC staining of mouse testis using 13886-1-AP (same clone as 13886-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13886-1-AP (SIAH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SIAH1 Polyclonal antibody, PBS Only

SIAH1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 13886-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13886-1-PBS targets SIAH1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4336

Product name: Recombinant human SIAH1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-282 aa of BC035562

Sequence: MSRQTATALPTGTSKCPPSQRVPALTGTTASNNDLASLFECPVCFDYVLPPILQCQSGHLVCSNCRPKLTCCPTCRGPLGSIRNLAMEKVANSVLFPCKYASSGCEITLPHTEKADHEELCEFRPYSCPCPGASCKWQGSLDAVMPHLMHQHKSITTLQGEDIVFLATDINLPGAVDWVMMQSCFGFHFMLVLEKQEKYDGHQQFFAIVQLIGTRKQAENFAYRLELNGHRRRLTWEATPRSIHEGIATAIMNSDCLVFDTSIAQLFAENGNLGINVTISMC

相同性解析による交差性が予測される生物種
Full Name seven in absentia homolog 1 (Drosophila)
Calculated molecular weight 282 aa, 31 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC035562
Gene Symbol SIAH1
Gene ID (NCBI) 6477
RRIDAB_10643374
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IUQ4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SIAH1, also named as HUMSIAH, Siah-1a and Siah-1S(21kd), belongs to the SINA (Seven in absentia) family. SIAH1 is known to cause indirect degradation of beta-catenin through formation of a complex with Siah-interacting protein (SIP), Skp1 and Ebi. SIAH1 can form heterodimer or homodimer such as Siah-1*Siah-1(70 or 62kd), Siah-1*Siah-1S(42kd) and Siah-1S*Siah-1S(51-56kd). Importantly, results from in vitro soft agar assay demonstrated that Siah-1S displays a promotion effect on cells tumorigenicity. (PMID:17420721)

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