Validation Data Gallery
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Tested Applications
| Positive WB detected in | K-562 cells, A549 cells, HEK-293T cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human colon cancer tissue, human prostate hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IP | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
27020-1-AP targets SHQ1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25559 Product name: Recombinant human SHQ1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-214 aa of BC017274 Sequence: MLTPAFDLSQDPDFLTIAIRVPYARVSEFDVYFEGSDFKFYAKPYFLRLTLPGRIVENGSEQGSYDADKGIFTIRLPKETPGQHFEGLNMLTALLAPRKSRTAKPLVEEIGASEIPEEVVDDEEFDWEIEQTPCEEVSESALNPQCHYGFGNLRSGVLQRLQDELSDVIDIKDPDFTPAAERRQKRLAAELAKFDPDHYLADFFEDEAIEQILK 相同性解析による交差性が予測される生物種 |
| Full Name | SHQ1 homolog (S. cerevisiae) |
| Calculated molecular weight | 593 aa, 67 kDa |
| Observed molecular weight | 70 kDa~80 kDa |
| GenBank accession number | BC017274 |
| Gene Symbol | SHQ1 |
| Gene ID (NCBI) | 55164 |
| RRID | AB_2880725 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6PI26 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SHQ1 antibody 27020-1-AP | Download protocol |
| IHC protocol for SHQ1 antibody 27020-1-AP | Download protocol |
| IP protocol for SHQ1 antibody 27020-1-AP | Download protocol |
| WB protocol for SHQ1 antibody 27020-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Hum Mol Genet The C-terminal extension of dyskerin is a dyskeratosis congenita mutational hotspot that modulates interaction with telomerase RNA and subcellular localization | ||
Exp Neurol Pathogenic SHQ1 variants result in disruptions to neuronal development and the dopaminergic pathway
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