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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using SHISA4 Polyclonal antibody (22197-1-AP)

    WB analysis of mouse brain using 22197-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22197-1-AP (SHISA4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SHISA4 Polyclonal antibody (22197-1-AP)

    IF Staining of HEK-293 using 22197-1-AP

    Immunofluorescent analysis of HEK-293 cells using 22197-1-AP (SHISA4 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

SHISA4 Polyclonal antibody

SHISA4 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 22197-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

C1orf40, hShisa4, Protein shisa 4, SHISA4, TMEM58, Transmembrane protein 58

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22197-1-AP targets SHISA4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHISA4 fusion protein Ag17357 相同性解析による交差性が予測される生物種
Full Name shisa homolog 4 (Xenopus laevis)
Calculated molecular weight 197 aa, 22 kDa
Observed molecular weight 25-27 kDa
GenBank accession numberBC104444
Gene Symbol SHISA4
Gene ID (NCBI) 149345
RRIDAB_2879024
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ96DD7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SHISA4 antibody 22197-1-APDownload protocol
IF protocol for SHISA4 antibody 22197-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols