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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SHISA4 Polyclonal antibody

SHISA4 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 22197-1-AP

Synonyms

C1orf40, hShisa4, Protein shisa 4, SHISA4, TMEM58, Transmembrane protein 58

Validation Data Gallery

Applications:
  • WB analysis of mouse brain using 22197-1-AP

    WB analysis of mouse brain using 22197-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22197-1-AP (SHISA4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 22197-1-AP

    IF Staining of HEK-293 using 22197-1-AP

    Immunofluorescent analysis of HEK-293 cells using 22197-1-AP (SHISA4 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inmouse brain tissue
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22197-1-AP is SHISA4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHISA4 fusion protein Ag17357 相同性解析による交差性が予測される生物種
Full Name shisa homolog 4 (Xenopus laevis)
Calculated molecular weight 197 aa, 22 kDa
Observed molecular weight 25-27 kDa
GenBank accession numberBC104444
Gene symbol SHISA4
Gene ID (NCBI) 149345
RRIDAB_2879024
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SHISA4 antibody 22197-1-APDownload protocol
IF protocol for SHISA4 antibody 22197-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols