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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Shh Recombinant antibody (83280-1-RR)

    WB analysis using 83280-1-RR (same clone as 83280-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83280-1-RR (SHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83280-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using Shh Recombinant antibody (83280-1-RR)

    IF Staining of NIH/3T3 using 83280-1-RR (same clone as 83280-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Shh antibody (83280-1-RR, Clone: 240057E7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83280-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83280-1-RR

    Affinity and Kinetic Characterization of 83280-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83280-1-RR against Mouse SHH were performed. The affinity constant is below 1 pM.

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Mouse Shh Recombinant antibody, PBS Only

Shh Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 83280-1-PBS
Clone No.240057E7

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

M100081, Hxl3, Hx, Hhg1, Dsh

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83280-1-PBS targets Shh in WB, IF/ICC, Indirect ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name sonic hedgehog
Calculated molecular weight48KD
Observed molecular weight19-50 kDa
GenBank accession numberNM_009170.3
Gene Symbol Shh
Gene ID (NCBI) 20423
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ62226
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.