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SHCBP1 Monoclonal antibody

SHCBP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68647-1-Ig
Clone No.1B2E12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

SHC SH2 domain-binding protein 1, PAL

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inPC-3 cells, U2OS cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68647-1-Ig targets SHCBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SHCBP1 fusion protein Ag17517 相同性解析による交差性が予測される生物種
Full Name SHC SH2-domain binding protein 1
Calculated molecular weight 672 aa, 76 kDa
Observed molecular weight76 kDa
GenBank accession numberBC030699
Gene Symbol SHCBP1
Gene ID (NCBI) 79801
RRIDAB_3670390
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8NEM2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SHCBP1 was initially identified as a potential gene associated with cell proliferation in fibroblasts. SHCBP1 exerts a crucial physiological function in normal tissue development. Chen et al. showed that SHCBP1 is an essential component of FGF signaling in neural progenitor cells. SHCBP1 is also upregulated during T cell proliferation and regulates CD4+ T cell effector function in vivo. Recently, SHCBP1 was shown to be closely associated with cancer development.

Protocols

Product Specific Protocols
WB protocol for SHCBP1 antibody 68647-1-IgDownload protocol
IHC protocol for SHCBP1 antibody 68647-1-IgDownload protocol
IF protocol for SHCBP1 antibody 68647-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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