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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SHCBP1 Monoclonal antibody

SHCBP1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B2E12

Cat no : 68647-1-Ig

Synonyms

FLJ22009, PAL, SHCBP1

Validation Data Gallery

Applications:
  • WB analysis using 68647-1-Ig

    WB analysis using 68647-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68647-1-Ig (SHCBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 68647-1-Ig

    IHC staining of human liver cancer using 68647-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68647-1-Ig (SHCBP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 68647-1-Ig

    IHC staining of human liver cancer using 68647-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68647-1-Ig (SHCBP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 68647-1-Ig

    IHC staining of human breast cancer using 68647-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68647-1-Ig (SHCBP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 68647-1-Ig

    IHC staining of human breast cancer using 68647-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68647-1-Ig (SHCBP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inPC-3 cells, U2OS cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

68647-1-Ig targets SHCBP1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SHCBP1 fusion protein Ag17517 相同性解析による交差性が予測される生物種
Full Name SHC SH2-domain binding protein 1
Calculated molecular weight 672 aa, 76 kDa
Observed molecular weight76 kDa
GenBank accession numberBC030699
Gene symbol SHCBP1
Gene ID (NCBI) 79801
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SHCBP1 was initially identified as a potential gene associated with cell proliferation in fibroblasts. SHCBP1 exerts a crucial physiological function in normal tissue development. Chen et al. showed that SHCBP1 is an essential component of FGF signaling in neural progenitor cells. SHCBP1 is also upregulated during T cell proliferation and regulates CD4+ T cell effector function in vivo. Recently, SHCBP1 was shown to be closely associated with cancer development.

Protocols

Product Specific Protocols
WB protocol for SHCBP1 antibody 68647-1-IgDownload protocol
IHC protocol for SHCBP1 antibody 68647-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols