Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of Neuro-2a using 12672-1-AP
Neuro-2a cells were subjected to SDS PAGE followed by western blot with 12672-1-AP (SHCBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 12672-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12672-1-AP (SHCBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 12672-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12672-1-AP (SHCBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 12672-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12672-1-AP (SHCBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 12672-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 12672-1-AP (SHCBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of Sp2/0 using 12672-1-AP
Sp2/0 cells were subjected to SDS PAGE followed by western blot with 12672-1-AP (SHCBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 12672-1-AP
IP result of anti-SHCBP1 (IP:12672-1-AP, 4ug; Detection:12672-1-AP 1:500) with Jurkat cells lysate 2400ug.
IHC staining of human liver cancer using 12672-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12672-1-AP (SHCBP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 12672-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12672-1-AP (SHCBP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 12672-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis using 12672-1-AP (SHCBP1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human tonsillitis using 12672-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis using 12672-1-AP (SHCBP1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 12672-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 12672-1-AP (SHCBP1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 12672-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 12672-1-AP (SHCBP1 antibody) at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

SHCBP1 Polyclonal antibody

SHCBP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 12672-1-AP

Publications(20)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

PAL, SHCBP1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (20)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, Jurkat cells, Sp2/0 cells, Neuro-2a cells, HepG2 cells, mouse thymus tissue
Positive IP detected in	Jurkat cells
Positive IHC detected in	human liver cancer tissue, human tonsillitis tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 8 publications below
WB	See 18 publications below
IHC	See 5 publications below
IF	See 4 publications below
IP	See 2 publications below
CoIP	See 2 publications below

Product Information

12672-1-AP targets SHCBP1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, canine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3355 Product name: Recombinant human SHCBP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 374-672 aa of BC030699 Sequence: NACFEGDTVIVCPGHYVVHGTFSIADSIELEGYGLPDDIVIEKRGKGDTFVDCTGADIKISGIKFVQHDAVEGILIVHRGKTTLENCVLQCETTGVTVRTSAEFLMKNSDLYGAKGAGIEIYPGSQCTLSDNGIHHCKEGILIKDFLDEHYDIPKISMVNNIIHNNEGYGVVLVKPTIFSDLQESAEDGTEENKALKIQTSGEPDVAERVDLEELIECATGKMELCARTDPSEQVEGNCEIVNELIAASTQKGQIKKKRLSELGITQADDNLMSQEMFVGIVGNQFKWNGKGSFGTFLF 相同性解析による交差性が予測される生物種
Full Name	SHC SH2-domain binding protein 1
Calculated molecular weight	672 aa, 76 kDa
Observed molecular weight	75-85 kDa
GenBank accession number	BC030699
Gene Symbol	SHCBP1
Gene ID (NCBI)	79801
RRID	AB_10639526
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8NEM2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SHCBP1 was initially identified as a potential gene associated with cell proliferation in fibroblasts. SHCBP1 exerts a crucial physiological function in normal tissue development. Chen et al. showed that SHCBP1 is an essential component of FGF signaling in neural progenitor cells. SHCBP1 is also upregulated during T cell proliferation and regulates CD4+ T cell effector function in vivo. Recently, SHCBP1 was shown to be closely associated with cancer development.

Protocols

Product Specific Protocols
IHC protocol for SHCBP1 antibody 12672-1-AP	Download protocol
IP protocol for SHCBP1 antibody 12672-1-AP	Download protocol
WB protocol for SHCBP1 antibody 12672-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancer Res Targeting SHCBP1 Inhibits Tumor Progression by Restoring Ciliogenesis in Ductal Carcinoma Authors - Wengui Shi View Article
mouse	WB	Nat Neurosci Impaired neurogenesis alters brain biomechanics in a neuroprogenitor-based genetic subtype of congenital hydrocephalus. Authors - Phan Q Duy View Article
human	WB	Mol Cell An interactome-based framework for DDB1- and CUL4-associated factor prioritization in targeted protein degradation. Authors - Satoshi Yamanaka View Article
human	IF,IHC, WB	J Exp Clin Cancer Res SHCBP1 promotes synovial sarcoma cell metastasis via targeting TGF-β1/Smad signaling pathway and is associated with poor prognosis. Authors - Changliang Peng KD Validated View Article
human	WB,IHC,IF,CoIP	MedComm (2020) SHCBP1 is a novel regulator of PLK1 phosphorylation and promotes prostate cancer bone metastasis Authors - Chen Tang KD Validated View Article
human	WB	Apoptosis SHCBP1 promotes cisplatin resistance of ovarian cancer through AKT/mTOR/Autophagy pathway Authors - Gonghua Qi KD Validated View Article

Validation Data Gallery

WB analysis of Neuro-2a using 12672-1-AP

WB analysis using 12672-1-AP

WB analysis using 12672-1-AP

WB analysis using 12672-1-AP

WB analysis of Jurkat using 12672-1-AP

WB analysis of Sp2/0 using 12672-1-AP

IP experiment of Jurkat using 12672-1-AP

IHC staining of human liver cancer using 12672-1-AP

IHC staining of human liver cancer using 12672-1-AP

IHC staining of human tonsillitis using 12672-1-AP

IHC staining of human tonsillitis using 12672-1-AP

IHC staining of human testis using 12672-1-AP

IHC staining of human testis using 12672-1-AP

SHCBP1 Polyclonal antibody

SHCBP1 Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Targeting SHCBP1 Inhibits Tumor Progression by Restoring Ciliogenesis in Ductal Carcinoma

Impaired neurogenesis alters brain biomechanics in a neuroprogenitor-based genetic subtype of congenital hydrocephalus.

An interactome-based framework for DDB1- and CUL4-associated factor prioritization in targeted protein degradation.

SHCBP1 promotes synovial sarcoma cell metastasis via targeting TGF-β1/Smad signaling pathway and is associated with poor prognosis.

SHCBP1 is a novel regulator of PLK1 phosphorylation and promotes prostate cancer bone metastasis

SHCBP1 promotes cisplatin resistance of ovarian cancer through AKT/mTOR/Autophagy pathway

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