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Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using SHC4 Recombinant antibody (85181-4-RR)

    WB analysis of fetal human brain using 85181-4-RR

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 85181-4-RR (SHC4 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SHC4 Recombinant monoclonal antibody (85181-4-RR)

    WB analysis using 85181-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85181-4-RR (SHC4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SHC4 Recombinant monoclonal antibody (85181-4-RR)

    IF Staining of HepG2 using 85181-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SHC4 antibody (85181-4-RR, Clone: 242805E3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using SHC4 Recombinant monoclonal antibody (85181-4-RR)

    IF Staining of SH-SY5Y using 85181-4-RR

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using SHC4 antibody (85181-4-RR, Clone: 242805E3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

SHC4 Recombinant monoclonal antibody

SHC4 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85181-4-RR
Clone No.242805E3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

242805E3, hShcD, Rai like protein, Rai-like protein, RaLP

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected infetal human brain tissue, A375 cells, HepG2 Cells
Positive IF/ICC detected inHepG2 cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85181-4-RR targets SHC4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag3336

Product name: Recombinant human SHC4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 324-630 aa of BC033907

Sequence: NGMAQDVISTIGQAFELRFKQYLKNPSLNTSCESEEVHIDSHAEEREDHEYYNEIPGKQPPVGGVSDMRIKVQATEQMAYCPIQCEKLCYLPGNSKCSSVYENCLEQSRAIGNVHPRGVQSQRGTSLLKHTCRVDLFDDPCYINTQALQSTPGSAGNQRSAQPLGSPWHCGKAPETVQPGATAQPASSHSLPHIKQQLWSEECYHGKLSRKAAESLLVKDGDFLVRESATSPGQYVLSGLQGGQAKHLLLVDPEGKVRTKDHVFDNVGHLIRYHMDNSLPIISSGSEVSLKQPVRKDNNPALLHSNK

相同性解析による交差性が予測される生物種
Full Name SHC (Src homology 2 domain containing) family, member 4
Calculated molecular weight 630 aa, 69 kDa
Observed molecular weight75 kDa
GenBank accession numberBC033907
Gene Symbol SHC4
Gene ID (NCBI) 399694
RRIDAB_3744080
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ6S5L8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SHC4 is a member of the SHC family and is characterized by a phosphotyrosine binding (PTB) domain and Src homology 2 (SH2) domain, which both bind to phosphorylated tyrosine residues on cell surface receptors and other signaling proteins. SHC family proteins contain the proline- and glycine-rich domains termed collagen homology 1 (CH1) and CH2 domains, which act as docking sites for certain proteins. Abnormal SHC4 expression promotes cell migration and invasion in melanoma, glioma, and hepatocellular carcinoma (PMID: 38052369).

Protocols

Product Specific Protocols
IF protocol for SHC4 antibody 85181-4-RRDownload protocol
WB protocol for SHC4 antibody 85181-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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