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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SHC4 Polyclonal antibody

SHC4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12641-1-AP

Synonyms

hShcD, Rai like protein, RaLP, SH2 domain protein C4, SHC transforming protein 4, SHC transforming protein D, SHC4, SHCD

Validation Data Gallery

Applications:
  • WB analysis using 12641-1-AP

    WB analysis using 12641-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 12641-1-AP

    WB analysis using 12641-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 12641-1-AP

    WB analysis of human brain using 12641-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 12641-1-AP

    WB analysis of HepG2 using 12641-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of human skeletal muscle using 12641-1-AP

    WB analysis of human skeletal muscle using 12641-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of A375 using 12641-1-AP

    WB analysis of A375 using 12641-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inSH-SY5Y cells, human brain tissue, HepG2 cells, human skeletal muscle tissue, mouse brain tissue, A375 cells, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Sample-dependent, check data in validation data gallery

Product Information

12641-1-AP targets SHC4 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHC4 fusion protein Ag3336 相同性解析による交差性が予測される生物種
Full Name SHC (Src homology 2 domain containing) family, member 4
Calculated molecular weight 630 aa, 69 kDa
Observed molecular weight 69 kDa
GenBank accession numberBC033907
Gene symbol SHC4
Gene ID (NCBI) 399694
RRIDAB_10643243
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SHC4 antibody 12641-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Clin Sci (Lond)

Down-regulation of the insulin signaling pathway by SHC may correlate with congenital heart disease in Chinese populations.

Authors - Zhiling Luo
View Article
humanIHC,IF

PeerJ

A new 12-gene diagnostic biomarker signature of melanoma revealed by integrated microarray analysis.

Authors - Liu Wanting W
View Article
mouse,humanWB,IHC,IF,CoIP

Cancer Lett

SHC4 orchestrates β-catenin pathway-mediated metastasis in triple-negative breast cancer by promoting Src kinase autophosphorylation

Authors - Wenjing Zhong
  • KD Validated
View Article