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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SHBG Polyclonal antibody (18200-1-AP)

    WB analysis using 18200-1-AP (same clone as 18200-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 18200-1-AP (SHBG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18200-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SHBG Polyclonal antibody (18200-1-AP)

    IHC staining of human liver using 18200-1-AP (same clone as 18200-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18200-1-AP (SHBG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18200-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SHBG Polyclonal antibody (18200-1-AP)

    IHC staining of mouse testis using 18200-1-AP (same clone as 18200-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18200-1-AP (SHBG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18200-1-PBS in a different storage buffer formulation.

SHBG Polyclonal antibody, PBS Only

SHBG Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 18200-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Testosterone-estradiol-binding globulin, Testis-specific androgen-binding protein, Sex steroid-binding protein, Sex steroid binding protein, Sex hormone-binding globulin

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18200-1-PBS targets SHBG in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12683

Product name: Recombinant human SHBG protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 30-402 aa of BC101785

Sequence: LRPVLPTQSAHDPPAVHLSNGPGQEPIAVMTFDLTKITKTSSSFEVRTWDPEGVIFYGDTNPKDDWFMLGLRDGRPEIQLHNHWAQLTVGAGPRLDDGRWHQVEVKMEGDSVLLEVDGEEVLRLRQVSGPLTSKRHPIMRIALGGLLFPASNLRLPLVPALDGCLRRDSWLDKQAEISASAPTSLRSCDVESNPGIFLPPGTQAEFNLRDIPQPHAEPWAFSLDLGLKQAAGSGHLLALGTPENPSWLSLHLQDQKVVLSSGSGPGLDLPLVLGLPLQLKLSMSRVVLSQGSKMKALALPPLGLAPLLNLWAKPQGRLFLGALPGEDSSTSFCLNGLWAQGQRLDVDQALNRSHEIWTHSCPQSPGNGTDASH

相同性解析による交差性が予測される生物種
Full Name sex hormone-binding globulin
Calculated molecular weight 402 aa, 44 kDa
Observed molecular weight 42-44 kDa
GenBank accession numberBC101785
Gene Symbol SHBG
Gene ID (NCBI) 6462
RRIDAB_2878517
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP04278
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SHBG (sex hormone-binding globulin), also known as androgen-binding protein (ABP), is the specific transport protein for sex steroid hormones in humans. SHBG is synthesized mainly by the liver and circulates in the blood, in form of homodimer with a single steroid-binding site per dimer. In addition to the plasma SHBG, a testis isoform expressed by Sortoli cells also exists due to the gene alternative splicing. Various bands (44-56 kDa) recognized by this antibody may represent variably glycosylated or different isoforms of SHBG.

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