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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SHB Polyclonal antibody (31967-1-AP)

    WB analysis using 31967-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31967-1-AP (SHB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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SHB Polyclonal antibody

SHB Polyclonal Antibody for WB, ELISA
Cat No. 31967-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

SH2 domain-containing adapter protein B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHUVEC cells, L02 cells, C2C12 cells, H9C2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31967-1-AP targets SHB in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHB fusion protein Ag35936 相同性解析による交差性が予測される生物種
Full Name Src homology 2 domain containing adaptor protein B
Calculated molecular weight55 kDa
Observed molecular weight55 kDa
GenBank accession numberBC136581
Gene Symbol SHB
Gene ID (NCBI) 6461
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ15464
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SH2 domain-containing adapter protein B (SHB) is an adaptor protein that operates downstream of tyrosine kinases. It is crucial in various cellular responses, including cell survival, differentiation, and proliferation (PMID: 29792386). SHB is involved in the modulation of focal adhesion kinase (FAK) signaling, which is important for regulating cell cycle progression in hematopoietic stem cells (PMID: 23528453). The protein has also been implicated in the progression of myeloproliferative disorders by affecting the activity of FAK and other signaling intermediates (PMID: 24952416).

Protocols

Product Specific Protocols
WB protocol for SHB antibody 31967-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols