Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 14626-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14626-1-AP (SHARPIN antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 14626-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14626-1-AP (SHARPIN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 14626-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 14626-1-AP (SHARPIN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 14626-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 14626-1-AP (SHARPIN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 14626-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14626-1-AP (SHARPIN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of Raji using 14626-1-AP
IP result of anti-SHARPIN (IP:14626-1-AP, 3ug; Detection:14626-1-AP 1:1500) with Raji cells lysate 2520ug.
IHC staining of human lymphoma using 14626-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14626-1-AP (SHARPIN Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 14626-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14626-1-AP (SHARPIN Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human small intestine using 14626-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14626-1-AP (SHARPIN Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human small intestine using 14626-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14626-1-AP (SHARPIN Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of Jurkat using 14626-1-AP
Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using SHARPIN antibody (14626-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Featured Product
KD/KO Validated

SHARPIN Polyclonal antibody

SHARPIN Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14626-1-AP

Publications(109)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

SIPL1, Shank-interacting protein-like 1, Shank-associated RH domain-interacting protein, PSEC0216, hSIPL1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (109)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, mouse brain tissue, mouse skeletal muscle tissue, Jurkat cells, Raji cells
Positive IP detected in	Raji cells
Positive IHC detected in	human lymphoma tissue, human small intestine tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Jurkat cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 12 publications below
WB	See 99 publications below
IHC	See 4 publications below
IF	See 3 publications below
IP	See 16 publications below
CoIP	See 3 publications below

Product Information

14626-1-AP targets SHARPIN in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6201 Product name: Recombinant human SHARPIN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-326 aa of BC025244 Sequence: MAPPAGGAAAAASDLGSAAVLLAVHAAVRPLGAGPDAEAQLRRLQLSADPERPGRFRLELLGAGPGAVNLEWPLESVSYTIRGPTQHELQPPPGGPGTLSLHFLNPQEAQRWAVLVRGATVEGQNGSKSNSPPALGPEACPVSLPSPPEASTLKGPPPEADLPRSPGNLTEREELAGSLARAIAGGDEKGAAQVAAVLAQHRVALSVQLQEACFPPGPIRLQVTLEDAASAASAASSAHVALQVHPHCTVAALQEQVFSELGFPPAVQRWVIGRCLCVPERSLASYGVRQDGDPAFLYLLSAPREAPAQLVLSFLHLHQCPRPPWL 相同性解析による交差性が予測される生物種
Full Name	SHANK-associated RH domain interactor
Calculated molecular weight	40 kDa
Observed molecular weight	40 kDa
GenBank accession number	BC025244
Gene Symbol	SHARPIN
Gene ID (NCBI)	81858
RRID	AB_2187734
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H0F6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SHARPIN is as a third component of the linear ubiquitin chain assembly complex (LUBAC), recruited to the CD40 and TNF receptor signaling complexes together with its other constituents, HOIL1 and HOIP(PMID:21455173).SHARPIN is enriched in the postsynaptic density and forms a complex with SHANK in heterologous cells and brain(PMID:11178875).It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for SHARPIN antibody 14626-1-AP	Download protocol
IHC protocol for SHARPIN antibody 14626-1-AP	Download protocol
IP protocol for SHARPIN antibody 14626-1-AP	Download protocol
WB protocol for SHARPIN antibody 14626-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nature OTULIN limits cell death and inflammation by deubiquitinating LUBAC. Authors - Klaus Heger View Article
mouse	WB,IP	Nature LUBAC is essential for embryogenesis by preventing cell death and enabling haematopoiesis. Authors - Nieves Peltzer View Article
human	WB,IP	Cell OTULIN Antagonizes LUBAC Signaling by Specifically Hydrolyzing Met1-Linked Polyubiquitin. Authors - Keusekotten Kirstin K View Article
human	WB	Cell The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity. Authors - Rune Busk Damgaard View Article
mouse	WB	Cancer Cell A Dual Role of Caspase-8 in Triggering and Sensing Proliferation-Associated DNA Damage, a Key Determinant of Liver Cancer Development. Authors - Yannick Boege View Article
mouse	WB	Nat Immunol Pellino3 ubiquitinates RIP2 and mediates Nod2-induced signaling and protective effects in colitis. Authors - Yang Shuo S View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 14626-1-AP

WB analysis of mouse skeletal muscle using 14626-1-AP

WB analysis of A549 using 14626-1-AP

WB analysis of Jurkat using 14626-1-AP

WB analysis of mouse brain using 14626-1-AP

IP experiment of Raji using 14626-1-AP

IHC staining of human lymphoma using 14626-1-AP

IHC staining of human lymphoma using 14626-1-AP

IHC staining of human small intestine using 14626-1-AP

IHC staining of human small intestine using 14626-1-AP

IF Staining of Jurkat using 14626-1-AP

SHARPIN Polyclonal antibody

SHARPIN Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

OTULIN limits cell death and inflammation by deubiquitinating LUBAC.

LUBAC is essential for embryogenesis by preventing cell death and enabling haematopoiesis.

OTULIN Antagonizes LUBAC Signaling by Specifically Hydrolyzing Met1-Linked Polyubiquitin.

The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity.

A Dual Role of Caspase-8 in Triggering and Sensing Proliferation-Associated DNA Damage, a Key Determinant of Liver Cancer Development.

Pellino3 ubiquitinates RIP2 and mediates Nod2-induced signaling and protective effects in colitis.